Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples

Kwang Soo Ahn, Jung Hoon Lee, Jong Myeon Park, Han Nim Choi, Wonyong Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Luminol chemiluminescence (CL) biosensor based on boronic acid modified gold substrate has been developed for the determination of glycated hemoglobin (HbA1c) in human blood samples. In order to selectively capture HbA1c in sample, carboxy-EG6-undecanethiol was self-assembled on a gold thin-film substrate, followed by covalent coupling of 3-aminophenyl boronic acid (3-APBA). The captured HbA1c containing four iron heme groups plays as a catalyst for luminol CL reaction in the presence of hydrogen peroxide, and thus the luminol CL response is linearly proportional to the amount of HbA1c captured on the biosensor surface. The present biosensor showed linear dynamic range of HbA1c from 2.5% to 17.0%, which well covers the clinically important concentration range. In addition, the present biosensor exhibited negligible response to interfering species such as hemoglobin, fructose, and sorbitol. The present HbA1c biosensor was applied to the determination of HbA1c in human blood samples and the results were well agreed with that obtained with a conventional method.

Original languageEnglish
Article numberBIOSD1501506
Pages (from-to)82-87
Number of pages6
JournalBiosensors and Bioelectronics
Volume75
DOIs
Publication statusPublished - 2016 Jan 15

Fingerprint

Luminol
Chemiluminescence
Hemoglobin
Glycosylated Hemoglobin A
Biosensing Techniques
Luminescence
Biosensors
Blood
Boronic Acids
Gold
Fructose
Acids
Sorbitol
Substrates
Heme
Hydrogen peroxide
Hydrogen Peroxide
Hemoglobins
Iron
Thin films

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Ahn, Kwang Soo ; Lee, Jung Hoon ; Park, Jong Myeon ; Choi, Han Nim ; Lee, Wonyong. / Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples. In: Biosensors and Bioelectronics. 2016 ; Vol. 75. pp. 82-87.
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Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples. / Ahn, Kwang Soo; Lee, Jung Hoon; Park, Jong Myeon; Choi, Han Nim; Lee, Wonyong.

In: Biosensors and Bioelectronics, Vol. 75, BIOSD1501506, 15.01.2016, p. 82-87.

Research output: Contribution to journalArticle

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