Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

Sang Kyou Lee, Bing Su, Stephen E. Maher, Alfred L.M. Bothwell

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Abstract

To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR β chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.

Original languageEnglish
Pages (from-to)2167-2176
Number of pages10
JournalEMBO Journal
Volume13
Issue number9
Publication statusPublished - 1994 May 20

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Lee, S. K., Su, B., Maher, S. E., & Bothwell, A. L. M. (1994). Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity. EMBO Journal, 13(9), 2167-2176.