Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

Sang Kyou Lee, Bing Su, Stephen E. Maher, Alfred L.M. Bothwell

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR β chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.

Original languageEnglish
Pages (from-to)2167-2176
Number of pages10
JournalEMBO Journal
Volume13
Issue number9
Publication statusPublished - 1994 May 20

Fingerprint

T-cells
Receptor Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-fyn
T-Cell Antigen Receptor
Protein-Tyrosine Kinases
T-Lymphocytes
Antigens
Ly Antigens
Chemical activation
Concanavalin A
Interleukin-1
Crosslinking
Clone Cells
RNA
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Lee, S. K., Su, B., Maher, S. E., & Bothwell, A. L. M. (1994). Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity. EMBO Journal, 13(9), 2167-2176.
Lee, Sang Kyou ; Su, Bing ; Maher, Stephen E. ; Bothwell, Alfred L.M. / Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity. In: EMBO Journal. 1994 ; Vol. 13, No. 9. pp. 2167-2176.
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Lee, SK, Su, B, Maher, SE & Bothwell, ALM 1994, 'Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity', EMBO Journal, vol. 13, no. 9, pp. 2167-2176.

Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity. / Lee, Sang Kyou; Su, Bing; Maher, Stephen E.; Bothwell, Alfred L.M.

In: EMBO Journal, Vol. 13, No. 9, 20.05.1994, p. 2167-2176.

Research output: Contribution to journalArticle

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