Lymphatic capillary invasion assay by a single cell migration chip

Yu Chih Chen, Steven G. Allen, Zhi Fen Wu, Sofia D. Merajver, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrated a single cell migration chip which can emulate cancer cell invasion in lymphatic capillaries through a migration channel with resistance choke points. Using a hydrodynamic capturing scheme based on the difference in flow resistance, the device allows for positioning single cells at the start of a migration channel. Different sizes of the resistance choke points in the migration channels are investigated to characterize the deformation capability of cells. To verify the migration platform, we used p38γ gene knockdown MDA-MB-231 (breast cancer) cells, which are known to show lower lymphatic metastasis in-vivo in a separate study. The reduction of the invasive ability in lymphatic capillaries was confirmed by the fabricated migration chip.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages968-970
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

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Electric inductors
Assays
Hydrodynamics
Genes
Cells

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chen, Y. C., Allen, S. G., Wu, Z. F., Merajver, S. D., & Yoon, E. (2012). Lymphatic capillary invasion assay by a single cell migration chip. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 968-970). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.
Chen, Yu Chih ; Allen, Steven G. ; Wu, Zhi Fen ; Merajver, Sofia D. ; Yoon, Euisik. / Lymphatic capillary invasion assay by a single cell migration chip. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 968-970 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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Chen, YC, Allen, SG, Wu, ZF, Merajver, SD & Yoon, E 2012, Lymphatic capillary invasion assay by a single cell migration chip. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 968-970, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

Lymphatic capillary invasion assay by a single cell migration chip. / Chen, Yu Chih; Allen, Steven G.; Wu, Zhi Fen; Merajver, Sofia D.; Yoon, Euisik.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 968-970 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen YC, Allen SG, Wu ZF, Merajver SD, Yoon E. Lymphatic capillary invasion assay by a single cell migration chip. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 968-970. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).