Lymphatic capillary invasion assay by a single cell migration chip

Yu Chih Chen, Steven G. Allen, Zhi Fen Wu, Sofia D. Merajver, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrated a single cell migration chip which can emulate cancer cell invasion in lymphatic capillaries through a migration channel with resistance choke points. Using a hydrodynamic capturing scheme based on the difference in flow resistance, the device allows for positioning single cells at the start of a migration channel. Different sizes of the resistance choke points in the migration channels are investigated to characterize the deformation capability of cells. To verify the migration platform, we used p38γ gene knockdown MDA-MB-231 (breast cancer) cells, which are known to show lower lymphatic metastasis in-vivo in a separate study. The reduction of the invasive ability in lymphatic capillaries was confirmed by the fabricated migration chip.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages968-970
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'Lymphatic capillary invasion assay by a single cell migration chip'. Together they form a unique fingerprint.

Cite this