M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas

Chanhee Lee, Joongyub Lee, Seung Ah Choi, Seung Ki Kim, Kyu Chang Wang, Sung Hye Park, Se Hoon Kim, Ji Yeoun Lee, Ji Hoon Phi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Recent progress in molecular analysis has advanced the understanding of medulloblastoma (MB) and is anticipated to facilitate management of the disease. MB is composed of 4 molecular subgroups: WNT, SHH, Group 3, and Group 4. Macrophages play a crucial role in the tumor microenvironment; however, the functional role of their activated phenotype (M1/M2) remains controversial. Herein, we investigate the correlation between tumor-associated macrophage (TAM) recruitment within the MB subgroups and prognosis. Methods: Molecular subgrouping was performed by a nanoString-based RNA assay on retrieved snap-frozen tissue samples. Immunohistochemistry (IHC) and immunofluorescence (IF) assays were performed on subgroup identified samples, and the number of polarized macrophages was quantified from IHC. Survival analyses were conducted on collected clinical data and quantified macrophage data. Results: TAM (M1/M2) recruitment in SHH MB was significantly higher compared to that in other subgroups. A Kaplan-Meier survival curve and multivariate Cox regression demonstrated that high M1 expressers showed worse overall survival (OS) and progression-free survival (PFS) than low M1 expressers in SHH MB, with relative risk (RR) values of 11.918 and 6.022, respectively. Conclusion: M1 rather than M2 correlates more strongly with worse outcome in SHH medulloblastoma.

Original languageEnglish
Article number535
JournalBMC cancer
Volume18
Issue number1
DOIs
Publication statusPublished - 2018 May 8

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Medulloblastoma
Macrophages
Immunohistochemistry
Tumor Microenvironment
Kaplan-Meier Estimate
Survival Analysis
Disease Management
Disease-Free Survival
Fluorescent Antibody Technique
Neoplasms
RNA
Phenotype

All Science Journal Classification (ASJC) codes

  • Oncology
  • Genetics
  • Cancer Research

Cite this

Lee, C., Lee, J., Choi, S. A., Kim, S. K., Wang, K. C., Park, S. H., ... Phi, J. H. (2018). M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas. BMC cancer, 18(1), [535]. https://doi.org/10.1186/s12885-018-4457-8
Lee, Chanhee ; Lee, Joongyub ; Choi, Seung Ah ; Kim, Seung Ki ; Wang, Kyu Chang ; Park, Sung Hye ; Kim, Se Hoon ; Lee, Ji Yeoun ; Phi, Ji Hoon. / M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas. In: BMC cancer. 2018 ; Vol. 18, No. 1.
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abstract = "Background: Recent progress in molecular analysis has advanced the understanding of medulloblastoma (MB) and is anticipated to facilitate management of the disease. MB is composed of 4 molecular subgroups: WNT, SHH, Group 3, and Group 4. Macrophages play a crucial role in the tumor microenvironment; however, the functional role of their activated phenotype (M1/M2) remains controversial. Herein, we investigate the correlation between tumor-associated macrophage (TAM) recruitment within the MB subgroups and prognosis. Methods: Molecular subgrouping was performed by a nanoString-based RNA assay on retrieved snap-frozen tissue samples. Immunohistochemistry (IHC) and immunofluorescence (IF) assays were performed on subgroup identified samples, and the number of polarized macrophages was quantified from IHC. Survival analyses were conducted on collected clinical data and quantified macrophage data. Results: TAM (M1/M2) recruitment in SHH MB was significantly higher compared to that in other subgroups. A Kaplan-Meier survival curve and multivariate Cox regression demonstrated that high M1 expressers showed worse overall survival (OS) and progression-free survival (PFS) than low M1 expressers in SHH MB, with relative risk (RR) values of 11.918 and 6.022, respectively. Conclusion: M1 rather than M2 correlates more strongly with worse outcome in SHH medulloblastoma.",
author = "Chanhee Lee and Joongyub Lee and Choi, {Seung Ah} and Kim, {Seung Ki} and Wang, {Kyu Chang} and Park, {Sung Hye} and Kim, {Se Hoon} and Lee, {Ji Yeoun} and Phi, {Ji Hoon}",
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Lee, C, Lee, J, Choi, SA, Kim, SK, Wang, KC, Park, SH, Kim, SH, Lee, JY & Phi, JH 2018, 'M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas', BMC cancer, vol. 18, no. 1, 535. https://doi.org/10.1186/s12885-018-4457-8

M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas. / Lee, Chanhee; Lee, Joongyub; Choi, Seung Ah; Kim, Seung Ki; Wang, Kyu Chang; Park, Sung Hye; Kim, Se Hoon; Lee, Ji Yeoun; Phi, Ji Hoon.

In: BMC cancer, Vol. 18, No. 1, 535, 08.05.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas

AU - Lee, Chanhee

AU - Lee, Joongyub

AU - Choi, Seung Ah

AU - Kim, Seung Ki

AU - Wang, Kyu Chang

AU - Park, Sung Hye

AU - Kim, Se Hoon

AU - Lee, Ji Yeoun

AU - Phi, Ji Hoon

PY - 2018/5/8

Y1 - 2018/5/8

N2 - Background: Recent progress in molecular analysis has advanced the understanding of medulloblastoma (MB) and is anticipated to facilitate management of the disease. MB is composed of 4 molecular subgroups: WNT, SHH, Group 3, and Group 4. Macrophages play a crucial role in the tumor microenvironment; however, the functional role of their activated phenotype (M1/M2) remains controversial. Herein, we investigate the correlation between tumor-associated macrophage (TAM) recruitment within the MB subgroups and prognosis. Methods: Molecular subgrouping was performed by a nanoString-based RNA assay on retrieved snap-frozen tissue samples. Immunohistochemistry (IHC) and immunofluorescence (IF) assays were performed on subgroup identified samples, and the number of polarized macrophages was quantified from IHC. Survival analyses were conducted on collected clinical data and quantified macrophage data. Results: TAM (M1/M2) recruitment in SHH MB was significantly higher compared to that in other subgroups. A Kaplan-Meier survival curve and multivariate Cox regression demonstrated that high M1 expressers showed worse overall survival (OS) and progression-free survival (PFS) than low M1 expressers in SHH MB, with relative risk (RR) values of 11.918 and 6.022, respectively. Conclusion: M1 rather than M2 correlates more strongly with worse outcome in SHH medulloblastoma.

AB - Background: Recent progress in molecular analysis has advanced the understanding of medulloblastoma (MB) and is anticipated to facilitate management of the disease. MB is composed of 4 molecular subgroups: WNT, SHH, Group 3, and Group 4. Macrophages play a crucial role in the tumor microenvironment; however, the functional role of their activated phenotype (M1/M2) remains controversial. Herein, we investigate the correlation between tumor-associated macrophage (TAM) recruitment within the MB subgroups and prognosis. Methods: Molecular subgrouping was performed by a nanoString-based RNA assay on retrieved snap-frozen tissue samples. Immunohistochemistry (IHC) and immunofluorescence (IF) assays were performed on subgroup identified samples, and the number of polarized macrophages was quantified from IHC. Survival analyses were conducted on collected clinical data and quantified macrophage data. Results: TAM (M1/M2) recruitment in SHH MB was significantly higher compared to that in other subgroups. A Kaplan-Meier survival curve and multivariate Cox regression demonstrated that high M1 expressers showed worse overall survival (OS) and progression-free survival (PFS) than low M1 expressers in SHH MB, with relative risk (RR) values of 11.918 and 6.022, respectively. Conclusion: M1 rather than M2 correlates more strongly with worse outcome in SHH medulloblastoma.

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DO - 10.1186/s12885-018-4457-8

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JO - BMC Cancer

JF - BMC Cancer

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