M2 phenotype microglia-derived cytokine stimulates proliferation and neuronal differentiation of endogenous stem cells in ischemic brain

Ja Yong Choi, Jong Youl Kim, Jae Young Kim, Joohyun Park, Won Taek Lee, Jong Eun Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Microglia play a key role in the immune response and inflammatory reaction that occurs in response to ischemic stroke. Activated microglia promote neuronal damage or protection in injured brain tissue. Extracellular signals polarize the microglia towards the M1/M2 phenotype. The M1/M2 phenotype microglia released pro- and anti-inflammatory cytokines which induce the activation of neural stem/progenitor cells (NSPCs). In this study, we investigated how the cytokines released by microglia affect the activation of NSPCs. First, we treated BV2 cells with a lipopolysaccharide (LPS; 20 ng/ml) for M1 phenotype microglia and interleukin-4 (IL-4; 20 ng/ml) for M2 phenotype microglia in BV2 cells. Mice were subjected to transient middle cerebral artery occlusion (tMCAO) for 1 h. In ex vivo, brain sections containing the subventricular zone (SVZ) were cultured in conditioned media of M1 and M2 phenotypeconditioned media for 3 d. We measured the expression of cytokines in the conditioned media by RT-PCR and ELISA. The M2 phenotype microglia-conditioned media led to the proliferation and neural differentiation of NSPCs in the ipsilateral SVZ after ischemic stroke. The RT-PCR and ELISA results showed that the expression of TGF-α mRNA was significantly higher in the M2 phenotype microglia-conditioned media. These data support that M2 phenotype microglia-derived TGF-α is one of the key factors to enhance proliferation and neural differntiation of NSPCs after ischemic stroke.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalExperimental Neurobiology
Volume26
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

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Microglia
Stem Cells
Cytokines
Phenotype
Brain
Neural Stem Cells
Conditioned Culture Medium
Lateral Ventricles
Stroke
Interleukin-4
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Middle Cerebral Artery Infarction
Lipopolysaccharides
Anti-Inflammatory Agents
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Choi, Ja Yong ; Kim, Jong Youl ; Kim, Jae Young ; Park, Joohyun ; Lee, Won Taek ; Lee, Jong Eun. / M2 phenotype microglia-derived cytokine stimulates proliferation and neuronal differentiation of endogenous stem cells in ischemic brain. In: Experimental Neurobiology. 2017 ; Vol. 26, No. 1. pp. 33-41.
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M2 phenotype microglia-derived cytokine stimulates proliferation and neuronal differentiation of endogenous stem cells in ischemic brain. / Choi, Ja Yong; Kim, Jong Youl; Kim, Jae Young; Park, Joohyun; Lee, Won Taek; Lee, Jong Eun.

In: Experimental Neurobiology, Vol. 26, No. 1, 01.01.2017, p. 33-41.

Research output: Contribution to journalArticle

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