Macelignan attenuates activations of mitogen-activated protein kinases and nuclear factor kappa B induced by lipopolysaccharide in microglial cells

Jinhua Ma, Yoo Kyeong Hwang, Woo Hyun Cho, Seol Heui Han, Jae-Kwan Hwang, Jung Soo Han

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A previous study showed that macelignan extracted from Myristica fragrans has anti-inflammatory properties using hippocampal neuronal and primary microglial cells. Subsequently, a study using animals with chronic lipopolysaccharide (LPS) infusion into the brain showed that oral treatments of macelignan reduced the hippocampal microglial activation and hippocampal-dependent spatial memory impairments induced by LPS. However, the molecular mechanisms responsible for the anti-inflammatory activity of macelignan have not been elucidated in the microglia. Therefore, the present study was conducted to determine if mitogen-activated protein kinase (MAPK) signaling and nuclear factor-kappa B (NF- κB) activities are related to the anti-inflammatory effects of macelignan on LPS-stimulated BV-2 microglial cells. The results show that macelignan suppresses both the phosphorylations of MAPKs and the degradation of inhibitory-kappa B (I κB α) and increases of nuclear NFκB in LPS-stimulated BV-2 microglial cells. These results suggest that macelignan has an anti-inflammatory effect on the affected brain through regulation of the inflammation through the MAPK signal pathway.

Original languageEnglish
Pages (from-to)1085-1090
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number6
DOIs
Publication statusPublished - 2009 Jun 1

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NF-kappa B
Mitogen-Activated Protein Kinases
Lipopolysaccharides
Anti-Inflammatory Agents
Myristica fragrans
Brain
Microglia
macelignan
Signal Transduction
Phosphorylation
Inflammation

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Pharmacology

Cite this

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title = "Macelignan attenuates activations of mitogen-activated protein kinases and nuclear factor kappa B induced by lipopolysaccharide in microglial cells",
abstract = "A previous study showed that macelignan extracted from Myristica fragrans has anti-inflammatory properties using hippocampal neuronal and primary microglial cells. Subsequently, a study using animals with chronic lipopolysaccharide (LPS) infusion into the brain showed that oral treatments of macelignan reduced the hippocampal microglial activation and hippocampal-dependent spatial memory impairments induced by LPS. However, the molecular mechanisms responsible for the anti-inflammatory activity of macelignan have not been elucidated in the microglia. Therefore, the present study was conducted to determine if mitogen-activated protein kinase (MAPK) signaling and nuclear factor-kappa B (NF- κB) activities are related to the anti-inflammatory effects of macelignan on LPS-stimulated BV-2 microglial cells. The results show that macelignan suppresses both the phosphorylations of MAPKs and the degradation of inhibitory-kappa B (I κB α) and increases of nuclear NFκB in LPS-stimulated BV-2 microglial cells. These results suggest that macelignan has an anti-inflammatory effect on the affected brain through regulation of the inflammation through the MAPK signal pathway.",
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Macelignan attenuates activations of mitogen-activated protein kinases and nuclear factor kappa B induced by lipopolysaccharide in microglial cells. / Ma, Jinhua; Hwang, Yoo Kyeong; Cho, Woo Hyun; Han, Seol Heui; Hwang, Jae-Kwan; Han, Jung Soo.

In: Biological and Pharmaceutical Bulletin, Vol. 32, No. 6, 01.06.2009, p. 1085-1090.

Research output: Contribution to journalArticle

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