Type I allergy is characterized by the release of granule-associated mediators, lipidderived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca 2+) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca2+ influx and the secretion of β-hexosaminidase, histamine, prostaglandin E2, and leukotriene C4; decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.
Bibliographical noteFunding Information:
This work was supported in part by the Yonsei Biomolecule Research Initiative of the Two-step Brain Korea 21 Project.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy