Macelignan inhibits histamine release and inflammatory mediator production in activated rat basophilic leukemia mast cells

Young Sun Han, Myung Suk Kim, Jae-Kwan Hwang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Type I allergy is characterized by the release of granule-associated mediators, lipidderived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca 2+) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca2+ influx and the secretion of β-hexosaminidase, histamine, prostaglandin E2, and leukotriene C4; decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.

Original languageEnglish
Pages (from-to)1723-1731
Number of pages9
JournalInflammation
Volume35
Issue number5
DOIs
Publication statusPublished - 2012 Oct 1

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Mast-Cell Leukemia
Histamine Release
Hypersensitivity
Myristica fragrans
Hexosaminidases
Arachidonate 5-Lipoxygenase
Leukotriene C4
Anti-Allergic Agents
Interleukin-13
JNK Mitogen-Activated Protein Kinases
Extracellular Signal-Regulated MAP Kinases
Calcimycin
Cyclooxygenase 2
Mitogen-Activated Protein Kinases
Chemokines
Dinoprostone
Mast Cells
Interleukin-4
Histamine
Acetates

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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abstract = "Type I allergy is characterized by the release of granule-associated mediators, lipidderived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca 2+) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca2+ influx and the secretion of β-hexosaminidase, histamine, prostaglandin E2, and leukotriene C4; decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.",
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Macelignan inhibits histamine release and inflammatory mediator production in activated rat basophilic leukemia mast cells. / Han, Young Sun; Kim, Myung Suk; Hwang, Jae-Kwan.

In: Inflammation, Vol. 35, No. 5, 01.10.2012, p. 1723-1731.

Research output: Contribution to journalArticle

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