Macelignan inhibits histamine release and inflammatory mediator production in activated rat basophilic leukemia mast cells

Young Sun Han, Myung Suk Kim, Jae Kwan Hwang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Type I allergy is characterized by the release of granule-associated mediators, lipidderived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca 2+) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca2+ influx and the secretion of β-hexosaminidase, histamine, prostaglandin E2, and leukotriene C4; decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.

Original languageEnglish
Pages (from-to)1723-1731
Number of pages9
JournalInflammation
Volume35
Issue number5
DOIs
Publication statusPublished - 2012 Oct 1

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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