Abstract
In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | BioFactors |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jul 2 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Clinical Biochemistry
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Macelignan protects HepG2 cells against tert-butylhydroperoxide-induced oxidative damage. / Sohn, Jong Hee; Han, Kyu Lee; Choo, Jeong Han; Hwang, Jae Kwan.
In: BioFactors, Vol. 29, No. 1, 02.07.2007, p. 1-10.Research output: Contribution to journal › Article
TY - JOUR
T1 - Macelignan protects HepG2 cells against tert-butylhydroperoxide-induced oxidative damage
AU - Sohn, Jong Hee
AU - Han, Kyu Lee
AU - Choo, Jeong Han
AU - Hwang, Jae Kwan
PY - 2007/7/2
Y1 - 2007/7/2
N2 - In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.
AB - In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.
UR - http://www.scopus.com/inward/record.url?scp=34250853473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250853473&partnerID=8YFLogxK
U2 - 10.1002/biof.5520290101
DO - 10.1002/biof.5520290101
M3 - Article
C2 - 17611289
AN - SCOPUS:34250853473
VL - 29
SP - 1
EP - 10
JO - BioFactors
JF - BioFactors
SN - 0951-6433
IS - 1
ER -