Macro-optical trapping for sample confinement in light sheet microscopy

Zhengyi Yang, Peeter Piksarv, David E.K. Ferrier, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.

Original languageEnglish
Pages (from-to)2778-2785
Number of pages8
JournalBiomedical Optics Express
Volume6
Issue number8
DOIs
Publication statusPublished - 2015 Jul 8

Fingerprint

Optical Tweezers
Microscopy
trapping
microscopy
Light
larvae
tobacco
Three-Dimensional Imaging
Plant Cells
bleaching
Sepharose
Tobacco
Larva
counters
Lasers
microscopes
traps
laser beams
damage
geometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Yang, Zhengyi ; Piksarv, Peeter ; Ferrier, David E.K. ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Macro-optical trapping for sample confinement in light sheet microscopy. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 8. pp. 2778-2785.
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Macro-optical trapping for sample confinement in light sheet microscopy. / Yang, Zhengyi; Piksarv, Peeter; Ferrier, David E.K.; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: Biomedical Optics Express, Vol. 6, No. 8, 08.07.2015, p. 2778-2785.

Research output: Contribution to journalArticle

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