Sepsis is a life-threatening condition caused by an uncontrolled response to bacterial infection. Impaired bactericidal activity in the host is directly associated with severe sepsis; however, the underlying regulatory mechanism(s) is largely unknown. Here, we show that MCL (macrophage C-type lectin) plays a crucial role in killing bacteria during Escherichia coli-induced peritonitis. MCL-deficient mice with E. coli-induced sepsis showed lower survival rates and reduced bacterial clearance when compared with control mice, despite similar levels of proinflammatory cytokine production. Although the ability of macrophages from MCL-deficient mice to kill bacteria was impaired, they showed normal phagocytic activity and production of reactive oxygen species. In addition, MCL-deficient macrophages showed defective phagosome maturation and phagosomal acidification after E. coli infection. Taken together, these results indicate that MCL plays an important role in host defense against E. coli infection by promoting phagosome maturation and acidification, thereby providing new insight into the role of MCL during pathogenesis of sepsis and offering new therapeutic options.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2017 Dec 2|
Bibliographical noteFunding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( NRF-2016R1D1A1B01015292 to L.K.K.), and by a faculty research grant from Yonsei University College of Medicine ( 6-2017-0037 to L.K.K.). This research was also supported by the Collaborative Genome Program for Fostering New Post-Genome industry through the National Research Foundation of Korea (NRF), funded by the Ministry of Science ICT and Future Planning (grant number: 2016M3C9A4921712 to Y.J.K.), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Science, IT & Future Planning ( NRF-2016R1A6A3A11934835 to W.-B.L.). Appendix A
© 2017 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology