Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens

Jihoon G. Yoon, Jin Seok Kang, Seung Yong Hwang, Jaewoo Song, Seok Hoon Jeong

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Two different methods - the semi-automated magnetic bead-based kit (SK, Stool DNA/RNA Purification kit®) and the manual membrane column-based kit (QS, QIAamp® DNA Stool Mini kit) - for purifying nucleic acids from clinical stool samples were compared and evaluated. The SK kit was more user-friendly than QS due to the reduced manual processing, partial automation, and short turnaround time with half cost. Furthermore, SK produced high yields in both DNA and RNA extractions but poor purity in RNA extraction. In the assessment of rotavirus and Clostridium difficile infection, both kits had equivalent or more sensitive performance compared with the standard method. Although SK showed some interference and inhibition in nucleic acid extraction, the performance, including the repeatability, linearity, analytical sensitivity, and matrix effect, was sufficient for routine clinical use.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalJournal of Microbiological Methods
Volume124
DOIs
Publication statusPublished - 2016 May 1

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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