Magnetic force nanoprobe for direct observation of audio frequency tonotopy of hair cells

Ji Wook Kim, Jae Hyun Lee, Ji Hyun Ma, Eunna Chung, Hongsuh Choi, Jinwoong Bok, Jinwoo Cheon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sound perception via mechano-sensation is a remarkably sensitive and fast transmission process, converting sound as a mechanical input to neural signals in a living organism. Although knowledge of auditory hair cell functions has advanced over the past decades, challenges remain in understanding their biomechanics, partly because of their biophysical complexity and the lack of appropriate probing tools. Most current studies of hair cells have been conducted in a relatively low-frequency range (<1000 Hz); therefore, fast kinetic study of hair cells has been difficult, even though mammalians have sound perception of 20 kHz or higher. Here, we demonstrate that the magnetic force nanoprobe (MFN) has superb spatiotemporal capabilities to mechanically stimulate spatially-targeted individual hair cells with a temporal resolution of up to 9 μs, which is equivalent to approximately 50 kHz; therefore, it is possible to investigate avian hair cell biomechanics at different tonotopic regions of the cochlea covering a full hearing frequency range of 50 to 5000 Hz. We found that the variation of the stimulation frequency and amplitude of hair bundles creates distinct mechanical responsive features along the tonotopic axis, where the kinetics of the hair bundle recovery motion exhibits unique frequency-dependent characteristics: basal, middle, and apical hair bundles can effectively respond at their respective ranges of frequency. We revealed that such recovery kinetics possesses two different time constants that are closely related to the passive and active motilities of hair cells. The use of MFN is critical for the kinetics study of free-standing hair cells in a spatiotemporally distinct tonotopic organization.

Original languageEnglish
Pages (from-to)3885-3891
Number of pages7
JournalNano letters
Volume16
Issue number6
DOIs
Publication statusPublished - 2016 Jun 8

Fingerprint

Nanoprobes
audio frequencies
hair
Cells
Kinetics
Biomechanics
Acoustic waves
auditory perception
biodynamics
bundles
kinetics
Recovery
Audition
frequency ranges
recovery
cochlea
locomotion
hearing
temporal resolution
stimulation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Kim, Ji Wook ; Lee, Jae Hyun ; Ma, Ji Hyun ; Chung, Eunna ; Choi, Hongsuh ; Bok, Jinwoong ; Cheon, Jinwoo. / Magnetic force nanoprobe for direct observation of audio frequency tonotopy of hair cells. In: Nano letters. 2016 ; Vol. 16, No. 6. pp. 3885-3891.
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Magnetic force nanoprobe for direct observation of audio frequency tonotopy of hair cells. / Kim, Ji Wook; Lee, Jae Hyun; Ma, Ji Hyun; Chung, Eunna; Choi, Hongsuh; Bok, Jinwoong; Cheon, Jinwoo.

In: Nano letters, Vol. 16, No. 6, 08.06.2016, p. 3885-3891.

Research output: Contribution to journalArticle

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