Magneto-microfluidic device for apoptotic cell separation

Hyun Seok Kim, Oh Taek Son, Kunhong Kim, Sang Hyeob Kim, Sung Lyul Maeng, Hyo Il Jung

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Separation of magnetically labeled cell has been a popular technique in cytometry. Normal Jurkat cells were treated with Chlorohexamide (CHX) and TRAIL to induce apoptosis. Apoptotic cells were treated with biotin functionalized fusion protein, C2A and streptavidin coated magnetic beads. The force balance between magnetic and drag force acting on the cells were calculated. The microfluidic device was designed accordingly. Results from our device were in a good agreement with those of the conventional flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.

Original languageEnglish
Pages416-418
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

Fingerprint

Microfluidics
Cells
Streptavidin
Cell death
Biotin
Drag
Fusion reactions
Apoptosis
Proteins
chlorohexamide

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, H. S., Son, O. T., Kim, K., Kim, S. H., Maeng, S. L., & Jung, H. I. (2006). Magneto-microfluidic device for apoptotic cell separation. 416-418. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.
Kim, Hyun Seok ; Son, Oh Taek ; Kim, Kunhong ; Kim, Sang Hyeob ; Maeng, Sung Lyul ; Jung, Hyo Il. / Magneto-microfluidic device for apoptotic cell separation. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.3 p.
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Kim, HS, Son, OT, Kim, K, Kim, SH, Maeng, SL & Jung, HI 2006, 'Magneto-microfluidic device for apoptotic cell separation', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 06/11/5 - 06/11/9 pp. 416-418.

Magneto-microfluidic device for apoptotic cell separation. / Kim, Hyun Seok; Son, Oh Taek; Kim, Kunhong; Kim, Sang Hyeob; Maeng, Sung Lyul; Jung, Hyo Il.

2006. 416-418 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conferencePaper

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AU - Kim, Hyun Seok

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AU - Kim, Sang Hyeob

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AU - Jung, Hyo Il

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AB - Separation of magnetically labeled cell has been a popular technique in cytometry. Normal Jurkat cells were treated with Chlorohexamide (CHX) and TRAIL to induce apoptosis. Apoptotic cells were treated with biotin functionalized fusion protein, C2A and streptavidin coated magnetic beads. The force balance between magnetic and drag force acting on the cells were calculated. The microfluidic device was designed accordingly. Results from our device were in a good agreement with those of the conventional flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.

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Kim HS, Son OT, Kim K, Kim SH, Maeng SL, Jung HI. Magneto-microfluidic device for apoptotic cell separation. 2006. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.