Regulation of genetic activity in single cells and tissues is pivotal to determine key cellular functions in current biomedicine, yet the conventional biochemical activators lack spatiotemporal precision due to the diffusion-mediated slow kinetics and nonselectivity. Here, we describe a magnetogenetic method for target-specific activation of a clustered regularly interspaced short palindromic repeats (CRISPR) system for the regulation of intracellular proteins. We used magnetomechanical force generated by the magnetic nanostructure to activate pre-encoded Piezo1, the mechanosensitive ion channel, on the target cell. The activated Piezo1 further triggers the intracellular Ca2+signaling pathway, inducing the pre-encoded genes to express genes of interest (GOIs), which is Cas9 protein for the CRISPR regulation of the target proteins. We demonstrated that this magnetogenetic CRISPR system successfully edits the target genome for both in vitro and pseudo-in vivo environments, providing a versatile magnetic platform for remote gene editing of animals with various size scales.
|Number of pages||8|
|Publication status||Published - 2022 Sept 28|
Bibliographical noteFunding Information:
CRE-SRE-NFAT with furin-modified human insulin containing plasmid was kindly gifted by Dr. Jeffrey M. Friedman (Rockefeller University, New York, NY, USA). CRISPR reporter plasmid and sgRNA- EGFR for the CRISPR reporter assay were kind gifts from Dr. Junho K. Hur (Kyung Hee University, Seoul, Korea). We thank Dr. Minsuk Kwak for help with and discussions of this research. This work was supported by the Institute for Basic Science (IBS-R026-D1). J.-H.L. is supported by the National Research Foundation of Korea (No. 2021R1A2C1093319).
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering