Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

Young Ki Hahn, Zongwen Jin, Joo H. Kang, Eunkeu Oh, Hak Sung Kim, Jung Tak Jang, Jinwoo Cheon, Hae Sim Park, Je Kyun Park

Research output: Contribution to conferencePaper

Abstract

This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).

Original languageEnglish
Pages320-322
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

Fingerprint

Allergens
Immunoglobulin E
Magnetic fields
Permanent magnets
Nanoparticles
Microstructure

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Hahn, Y. K., Jin, Z., Kang, J. H., Oh, E., Kim, H. S., Jang, J. T., ... Park, J. K. (2006). Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples. 320-322. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.
Hahn, Young Ki ; Jin, Zongwen ; Kang, Joo H. ; Oh, Eunkeu ; Kim, Hak Sung ; Jang, Jung Tak ; Cheon, Jinwoo ; Park, Hae Sim ; Park, Je Kyun. / Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.3 p.
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abstract = "This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).",
author = "Hahn, {Young Ki} and Zongwen Jin and Kang, {Joo H.} and Eunkeu Oh and Kim, {Hak Sung} and Jang, {Jung Tak} and Jinwoo Cheon and Park, {Hae Sim} and Park, {Je Kyun}",
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Hahn, YK, Jin, Z, Kang, JH, Oh, E, Kim, HS, Jang, JT, Cheon, J, Park, HS & Park, JK 2006, 'Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 06/11/5 - 06/11/9 pp. 320-322.

Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples. / Hahn, Young Ki; Jin, Zongwen; Kang, Joo H.; Oh, Eunkeu; Kim, Hak Sung; Jang, Jung Tak; Cheon, Jinwoo; Park, Hae Sim; Park, Je Kyun.

2006. 320-322 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conferencePaper

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T1 - Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

AU - Hahn, Young Ki

AU - Jin, Zongwen

AU - Kang, Joo H.

AU - Oh, Eunkeu

AU - Kim, Hak Sung

AU - Jang, Jung Tak

AU - Cheon, Jinwoo

AU - Park, Hae Sim

AU - Park, Je Kyun

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Y1 - 2006/1/1

N2 - This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).

AB - This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).

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Hahn YK, Jin Z, Kang JH, Oh E, Kim HS, Jang JT et al. Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples. 2006. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.