For efficient labeling and tracking via magnetic resonance (MR) imaging of human mesenchymal stem cells (h-MSCs), magnetic labeling agents must be responsive to an external magnetic field. Thus, we developed ultrasensitive magnetoplex as a magnetic labeling agent composed of PEGylated MnFe 2O4 nanocrystals (PMNCs) and polycationics (poly-L-lysine, PLL) for efficient labeling of the h- MSCs and monitoring of the transplanted h-MSCs for a long term. PMNCs were prepared by nanoemulsion methods composed of MnFe2O4 nanocrystals (MNCs) and amphiphilic polymers (mPEG-dodecanoic acid). The prepared PMNCs exhibited excellent biocompatibility and their polycationic complexes (PMNCs/ PLL) demonstrated remarkable sensitivity compared with magnetic iron oxide nanoparticles (MION)/PLL or Ferumoxides/PLL. Furthermore, PMNCs demonstrated the potentials for novel diagnostic and therapeutic strategies with potential applications in various biomedical fields.
Bibliographical noteFunding Information:
Acknowledgments This study was supported by the Korea Science and Engineering Foundation (KOSEF) (R15-2004-024-00000-0, M10755020001-07N5502-00110, and R01-2006-000-10023-0) and a grant from the National R&D Program for Cancer Control, Korea Ministry of Health and Welfare (0620190-1).
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics
- Modelling and Simulation
- Materials Science(all)
- Condensed Matter Physics