Maintenance of anti-inflammatory cytokines and reduction of glial activation in the ischemic hippocampal CA1 region preconditioned with lipopolysaccharide

Jia Tian Yu, Choong Hyun Lee, Ki Yeon Yoo, Jung Hoon Choi, Hua Li, Ok Kyu Park, Bingchun Yan, In Koo Hwang, Young-Guen Kwon, Young Myeong Kim, Moo Ho Won

Research output: Contribution to journalArticle

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Abstract

Lipopolysaccharide (LPS) induces a strong immune response, and pretreatment with low dose of LPS suppresses the production of proinflammatory mediators. In the present study, we investigated the effect of LPS preconditioning on the delayed neuronal death in the gerbil hippocampal CA1 region after 5 min of transient cerebral ischemia. LPS preconditioning showed neuroprotective effects against ischemic damage in the hippocampal CA1 region after ischemic insult: About 92% of neurons in the CA1 region survived in the LPS-treated ischemia group. LPS preconditioning maintained anti-inflammatory cytokines, such as interleukin (IL)-4 and IL-13, in pyramidal neurons in the CA1 region after ischemia/reperfusion. In addition, IL-4 and IL-13 protein levels in the CA1 region of the LPS-treated ischemia group were similar to the vehicle-treated sham group. We found that reactive gliosis was markedly attenuated in the CA1 region of the LPS-treated ischemia group compared to the vehicle-treated ischemia group using immunohistochemistry of glial fibrillary acidic protein for astrocytes, and ionized calcium-binding adapter molecule 1 and isolectin B4 for microglia. These results indicate that LPS preconditioning may provide neuroprotection in the ischemic hippocampal CA1 region via maintenance of anti-inflammatory cytokines and suppression of glial activation.

Original languageEnglish
Pages (from-to)69-78
Number of pages10
JournalJournal of the Neurological Sciences
Volume296
Issue number1-2
DOIs
Publication statusPublished - 2010 Sep 15

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Hippocampal CA1 Region
Neuroglia
Lipopolysaccharides
Anti-Inflammatory Agents
Maintenance
Cytokines
Ischemia
Interleukin-13
Interleukin-4
Gliosis
Gerbillinae
Pyramidal Cells
Glial Fibrillary Acidic Protein
Transient Ischemic Attack
Microglia
Neuroprotective Agents
Lectins
Astrocytes
Reperfusion
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Yu, Jia Tian ; Lee, Choong Hyun ; Yoo, Ki Yeon ; Choi, Jung Hoon ; Li, Hua ; Park, Ok Kyu ; Yan, Bingchun ; Hwang, In Koo ; Kwon, Young-Guen ; Kim, Young Myeong ; Won, Moo Ho. / Maintenance of anti-inflammatory cytokines and reduction of glial activation in the ischemic hippocampal CA1 region preconditioned with lipopolysaccharide. In: Journal of the Neurological Sciences. 2010 ; Vol. 296, No. 1-2. pp. 69-78.
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Maintenance of anti-inflammatory cytokines and reduction of glial activation in the ischemic hippocampal CA1 region preconditioned with lipopolysaccharide. / Yu, Jia Tian; Lee, Choong Hyun; Yoo, Ki Yeon; Choi, Jung Hoon; Li, Hua; Park, Ok Kyu; Yan, Bingchun; Hwang, In Koo; Kwon, Young-Guen; Kim, Young Myeong; Won, Moo Ho.

In: Journal of the Neurological Sciences, Vol. 296, No. 1-2, 15.09.2010, p. 69-78.

Research output: Contribution to journalArticle

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