MAL and TMEM220 are novel DNA methylation markers in human gastric cancer

Boram Choi, Tae Su Han, Jimin Min, Keun Hur, Sun Min Lee, Hyuk Joon Lee, Young Joon Kim, Han Kwang Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalBiomarkers
Volume22
Issue number1
DOIs
Publication statusPublished - 2017 Jan 2

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Methylation
DNA Methylation
Genetic Markers
Stomach Neoplasms
Tissue
Genes
decitabine
Cells
Polymerase chain reaction
RNA-Directed DNA Polymerase
Gene expression
Cell Line
Gastric Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
Real-Time Polymerase Chain Reaction
Mucous Membrane
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

Choi, B., Han, T. S., Min, J., Hur, K., Lee, S. M., Lee, H. J., ... Yang, H. K. (2017). MAL and TMEM220 are novel DNA methylation markers in human gastric cancer. Biomarkers, 22(1), 35-44. https://doi.org/10.1080/1354750X.2016.1201542
Choi, Boram ; Han, Tae Su ; Min, Jimin ; Hur, Keun ; Lee, Sun Min ; Lee, Hyuk Joon ; Kim, Young Joon ; Yang, Han Kwang. / MAL and TMEM220 are novel DNA methylation markers in human gastric cancer. In: Biomarkers. 2017 ; Vol. 22, No. 1. pp. 35-44.
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Choi, B, Han, TS, Min, J, Hur, K, Lee, SM, Lee, HJ, Kim, YJ & Yang, HK 2017, 'MAL and TMEM220 are novel DNA methylation markers in human gastric cancer', Biomarkers, vol. 22, no. 1, pp. 35-44. https://doi.org/10.1080/1354750X.2016.1201542

MAL and TMEM220 are novel DNA methylation markers in human gastric cancer. / Choi, Boram; Han, Tae Su; Min, Jimin; Hur, Keun; Lee, Sun Min; Lee, Hyuk Joon; Kim, Young Joon; Yang, Han Kwang.

In: Biomarkers, Vol. 22, No. 1, 02.01.2017, p. 35-44.

Research output: Contribution to journalArticle

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AU - Lee, Hyuk Joon

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N2 - Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

AB - Context: Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide. Objective: To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. Materials and methods: Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis. Results:MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2′-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. Discussion and conclusion: These loci may serve as novel methylation markers for patients with GC.

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