Abstract
In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.
| Original language | English |
|---|---|
| Article number | 275102 |
| Journal | Nanotechnology |
| Volume | 25 |
| Issue number | 27 |
| DOIs | |
| Publication status | Published - 2014 Jul 11 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Bioengineering
- Chemistry(all)
- Electrical and Electronic Engineering
- Mechanical Engineering
- Mechanics of Materials
- Materials Science(all)
- Medicine(all)
Cite this
}
Maleimidyl magnetic nanoplatform for facile molecular MRI. / Heo, Dan; Lee, Eugene; Ku, Minhee; Hwang, Seungyeon; Kim, Bongjune; Park, Yeonji; Han Lee, Young; Huh, yongmin; Haam, Seungjoo; Cheong, Jae Ho; Yang, Jaemoon; Suh, Jinsuck.
In: Nanotechnology, Vol. 25, No. 27, 275102, 11.07.2014.Research output: Contribution to journal › Article
TY - JOUR
T1 - Maleimidyl magnetic nanoplatform for facile molecular MRI
AU - Heo, Dan
AU - Lee, Eugene
AU - Ku, Minhee
AU - Hwang, Seungyeon
AU - Kim, Bongjune
AU - Park, Yeonji
AU - Han Lee, Young
AU - Huh, yongmin
AU - Haam, Seungjoo
AU - Cheong, Jae Ho
AU - Yang, Jaemoon
AU - Suh, Jinsuck
PY - 2014/7/11
Y1 - 2014/7/11
N2 - In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.
AB - In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.
UR - http://www.scopus.com/inward/record.url?scp=84903287361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903287361&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/25/27/275102
DO - 10.1088/0957-4484/25/27/275102
M3 - Article
C2 - 24960226
AN - SCOPUS:84903287361
VL - 25
JO - Nanotechnology
JF - Nanotechnology
SN - 0957-4484
IS - 27
M1 - 275102
ER -