Maleimidyl magnetic nanoplatform for facile molecular MRI

Dan Heo, Eugene Lee, Minhee Ku, Seungyeon Hwang, Bongjune Kim, Yeonji Park, Young Han Lee, yongmin Huh, Seungjoo Haam, Jae Ho Cheong, Jaemoon Yang, Jinsuck Suh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.

Original languageEnglish
Article number275102
JournalNanotechnology
Volume25
Issue number27
DOIs
Publication statusPublished - 2014 Jul 11

Fingerprint

Magnetic resonance imaging
Molecular Probes
Biomarkers
Peptide Aptamers
Molecular Imaging
Sulfhydryl Compounds
Heterografts
Neoplasms
Molecular imaging
Cell Line
Cytotoxicity
Peptides
Cells
maleimide

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)
  • Medicine(all)

Cite this

Heo, Dan ; Lee, Eugene ; Ku, Minhee ; Hwang, Seungyeon ; Kim, Bongjune ; Park, Yeonji ; Han Lee, Young ; Huh, yongmin ; Haam, Seungjoo ; Cheong, Jae Ho ; Yang, Jaemoon ; Suh, Jinsuck. / Maleimidyl magnetic nanoplatform for facile molecular MRI. In: Nanotechnology. 2014 ; Vol. 25, No. 27.
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Heo, D, Lee, E, Ku, M, Hwang, S, Kim, B, Park, Y, Han Lee, Y, Huh, Y, Haam, S, Cheong, JH, Yang, J & Suh, J 2014, 'Maleimidyl magnetic nanoplatform for facile molecular MRI', Nanotechnology, vol. 25, no. 27, 275102. https://doi.org/10.1088/0957-4484/25/27/275102

Maleimidyl magnetic nanoplatform for facile molecular MRI. / Heo, Dan; Lee, Eugene; Ku, Minhee; Hwang, Seungyeon; Kim, Bongjune; Park, Yeonji; Han Lee, Young; Huh, yongmin; Haam, Seungjoo; Cheong, Jae Ho; Yang, Jaemoon; Suh, Jinsuck.

In: Nanotechnology, Vol. 25, No. 27, 275102, 11.07.2014.

Research output: Contribution to journalArticle

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AU - Han Lee, Young

AU - Huh, yongmin

AU - Haam, Seungjoo

AU - Cheong, Jae Ho

AU - Yang, Jaemoon

AU - Suh, Jinsuck

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Heo D, Lee E, Ku M, Hwang S, Kim B, Park Y et al. Maleimidyl magnetic nanoplatform for facile molecular MRI. Nanotechnology. 2014 Jul 11;25(27). 275102. https://doi.org/10.1088/0957-4484/25/27/275102