Maleimidyl magnetic nanoplatform for facile molecular MRI

Dan Heo; Eugene Lee; Minhee Ku; Seungyeon Hwang; Bongjune Kim; Yeonji Park; Young Han Lee; Yong Min Huh; Seungjoo Haam; Jae Ho Cheong; Jaemoon Yang; Jin Suck Suh

doi:10.1088/0957-4484/25/27/275102

Maleimidyl magnetic nanoplatform for facile molecular MRI

Dan Heo, Eugene Lee, Minhee Ku, Seungyeon Hwang, Bongjune Kim, Yeonji Park, Young Han Lee, Yong Min Huh, Seungjoo Haam, Jae Ho Cheong, Jaemoon Yang, Jin Suck Suh

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Abstract

In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.

Original language	English
Article number	275102
Journal	Nanotechnology
Volume	25
Issue number	27
DOIs	https://doi.org/10.1088/0957-4484/25/27/275102
Publication status	Published - 2014 Jul 11

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All Science Journal Classification (ASJC) codes

Bioengineering
Chemistry(all)
Materials Science(all)
Mechanics of Materials
Mechanical Engineering
Electrical and Electronic Engineering

Cite this

Heo, D., Lee, E., Ku, M., Hwang, S., Kim, B., Park, Y., Han Lee, Y., Huh, Y. M., Haam, S., Cheong, J. H., Yang, J., & Suh, J. S. (2014). Maleimidyl magnetic nanoplatform for facile molecular MRI. Nanotechnology, 25(27), [275102]. https://doi.org/10.1088/0957-4484/25/27/275102

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abstract = "In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.",

author = "Dan Heo and Eugene Lee and Minhee Ku and Seungyeon Hwang and Bongjune Kim and Yeonji Park and {Han Lee}, Young and Huh, {Yong Min} and Seungjoo Haam and Cheong, {Jae Ho} and Jaemoon Yang and Suh, {Jin Suck}",

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AU - Han Lee, Young

AU - Huh, Yong Min

AU - Haam, Seungjoo

AU - Cheong, Jae Ho

AU - Yang, Jaemoon

AU - Suh, Jin Suck

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10.1088/0957-4484/25/27/275102