Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

Yuko Ishikawa, Hiroko Ida-Yonemochi, Hironobu Suzuki, Kuniko Nakakura-Ohshima, Han Sung Jung, Masaki J. Honda, Yumiko Ishii, Nobukazu Watanabe, Hayato Ohshima

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76%) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs.

Original languageEnglish
Pages (from-to)227-241
Number of pages15
JournalHistochemistry and cell biology
Volume134
Issue number3
DOIs
Publication statusPublished - 2010 Sep 1

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Dental Pulp
Bromodeoxyuridine
Tooth
Wounds and Injuries
Odontoblasts
Tooth Injuries
Adult Stem Cells
Side-Population Cells
Mesenchymal Stromal Cells
Thymidine

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Ishikawa, Yuko ; Ida-Yonemochi, Hiroko ; Suzuki, Hironobu ; Nakakura-Ohshima, Kuniko ; Jung, Han Sung ; Honda, Masaki J. ; Ishii, Yumiko ; Watanabe, Nobukazu ; Ohshima, Hayato. / Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries. In: Histochemistry and cell biology. 2010 ; Vol. 134, No. 3. pp. 227-241.
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abstract = "Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76{\%}) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs.",
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Ishikawa, Y, Ida-Yonemochi, H, Suzuki, H, Nakakura-Ohshima, K, Jung, HS, Honda, MJ, Ishii, Y, Watanabe, N & Ohshima, H 2010, 'Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries', Histochemistry and cell biology, vol. 134, no. 3, pp. 227-241. https://doi.org/10.1007/s00418-010-0727-5

Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries. / Ishikawa, Yuko; Ida-Yonemochi, Hiroko; Suzuki, Hironobu; Nakakura-Ohshima, Kuniko; Jung, Han Sung; Honda, Masaki J.; Ishii, Yumiko; Watanabe, Nobukazu; Ohshima, Hayato.

In: Histochemistry and cell biology, Vol. 134, No. 3, 01.09.2010, p. 227-241.

Research output: Contribution to journalArticle

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T1 - Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

AU - Ishikawa, Yuko

AU - Ida-Yonemochi, Hiroko

AU - Suzuki, Hironobu

AU - Nakakura-Ohshima, Kuniko

AU - Jung, Han Sung

AU - Honda, Masaki J.

AU - Ishii, Yumiko

AU - Watanabe, Nobukazu

AU - Ohshima, Hayato

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