Abstract
An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.
Original language | English |
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Pages (from-to) | 2781-2786 |
Number of pages | 6 |
Journal | Advanced Materials |
Volume | 21 |
Issue number | 27 |
DOIs | |
Publication status | Published - 2009 Jul 20 |
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering