Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells

Ying Mei, Sharon Gerecht, Michael Taylor, Andrew J. Urquhart, Said R. Bogatyrev, Seung-Woo Cho, Martyn C. Davies, Morgan R. Alexander, Robert S. Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.

Original languageEnglish
Pages (from-to)2781-2786
Number of pages6
JournalAdvanced Materials
Volume21
Issue number27
DOIs
Publication statusPublished - 2009 Jul 20

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Biocompatible Materials
Stem cells
Biomaterials
Monomers
Proteins
Polymers
Adhesion
Hydrophobicity
Crosslinking
Robotics
Copolymers
acrylic acid

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Mei, Y., Gerecht, S., Taylor, M., Urquhart, A. J., Bogatyrev, S. R., Cho, S-W., ... Anderson, D. G. (2009). Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells. Advanced Materials, 21(27), 2781-2786. https://doi.org/10.1002/adma.200803184
Mei, Ying ; Gerecht, Sharon ; Taylor, Michael ; Urquhart, Andrew J. ; Bogatyrev, Said R. ; Cho, Seung-Woo ; Davies, Martyn C. ; Alexander, Morgan R. ; Langer, Robert S. ; Anderson, Daniel G. / Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells. In: Advanced Materials. 2009 ; Vol. 21, No. 27. pp. 2781-2786.
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Mei, Y, Gerecht, S, Taylor, M, Urquhart, AJ, Bogatyrev, SR, Cho, S-W, Davies, MC, Alexander, MR, Langer, RS & Anderson, DG 2009, 'Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells', Advanced Materials, vol. 21, no. 27, pp. 2781-2786. https://doi.org/10.1002/adma.200803184

Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells. / Mei, Ying; Gerecht, Sharon; Taylor, Michael; Urquhart, Andrew J.; Bogatyrev, Said R.; Cho, Seung-Woo; Davies, Martyn C.; Alexander, Morgan R.; Langer, Robert S.; Anderson, Daniel G.

In: Advanced Materials, Vol. 21, No. 27, 20.07.2009, p. 2781-2786.

Research output: Contribution to journalArticle

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