Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells

Ying Mei, Sharon Gerecht, Michael Taylor, Andrew J. Urquhart, Said R. Bogatyrev, Seung Woo Cho, Martyn C. Davies, Morgan R. Alexander, Robert S. Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.

Original languageEnglish
Pages (from-to)2781-2786
Number of pages6
JournalAdvanced Materials
Volume21
Issue number27
DOIs
Publication statusPublished - 2009 Jul 20

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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    Mei, Y., Gerecht, S., Taylor, M., Urquhart, A. J., Bogatyrev, S. R., Cho, S. W., Davies, M. C., Alexander, M. R., Langer, R. S., & Anderson, D. G. (2009). Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells. Advanced Materials, 21(27), 2781-2786. https://doi.org/10.1002/adma.200803184