Abstract
An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.
| Original language | English |
|---|---|
| Pages (from-to) | 2781-2786 |
| Number of pages | 6 |
| Journal | Advanced Materials |
| Volume | 21 |
| Issue number | 27 |
| DOIs | |
| Publication status | Published - 2009 Jul 20 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering
Cite this
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Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells. / Mei, Ying; Gerecht, Sharon; Taylor, Michael; Urquhart, Andrew J.; Bogatyrev, Said R.; Cho, Seung-Woo; Davies, Martyn C.; Alexander, Morgan R.; Langer, Robert S.; Anderson, Daniel G.
In: Advanced Materials, Vol. 21, No. 27, 20.07.2009, p. 2781-2786.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mapping the interactions among biomaterials,adsorbed proteins, and human embryonic stem cells
AU - Mei, Ying
AU - Gerecht, Sharon
AU - Taylor, Michael
AU - Urquhart, Andrew J.
AU - Bogatyrev, Said R.
AU - Cho, Seung-Woo
AU - Davies, Martyn C.
AU - Alexander, Morgan R.
AU - Langer, Robert S.
AU - Anderson, Daniel G.
PY - 2009/7/20
Y1 - 2009/7/20
N2 - An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.
AB - An array of 496 different biomaterials was synthesized and studied to examine the attachment of human embryonic-body (hEB) cells, partially differentiated human differentiated human embryonic-body (hEB) cells. Acrylate-based biomaterial arrays were fabricated using a robotic stage modified with a long-wave UV source and 22 acrylate monomers were chosen to maximize the diversity of their hydrophobicity/hycrophilicity and crosslinking density. During the high-resolution scan, only the polymer spots were scanned, and the intensities of fluorescently labeled cellular markers on the polymer spots enabled a quantitative measurement of cellular behavior. The capacity of the copolymers to influence the cell attachment depends on the major and minor monomers. Saturated cell attachment is observed on the high-adhesion major monomers such as 4, 8, 13, 14, and 15, while a lower level of cell attachment is found on low-adhesion spots containing monomers such as 3 and 16.
UR - http://www.scopus.com/inward/record.url?scp=67651091463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67651091463&partnerID=8YFLogxK
U2 - 10.1002/adma.200803184
DO - 10.1002/adma.200803184
M3 - Article
AN - SCOPUS:67651091463
VL - 21
SP - 2781
EP - 2786
JO - Advanced Materials
JF - Advanced Materials
SN - 0935-9648
IS - 27
ER -