Matrix metalloproteinase 9-activatable peptide-conjugated hydrogel-based fluorogenic intraocular-lens sensor

Moo Kwang Shin, Yong Woo Ji, Chae Eun Moon, Hyo Lee, Byunghoon Kang, Woo Seok Jinn, Jisun Ki, Byunggeol Mun, Myeong Hoon Kim, Hyung Keun Lee, Seungjoo Haam

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The eye is an extension of the central nervous system (CNS) and contains aqueous humor (AH), which is a fluid rich in biomolecules secreted from intraocular tissues; thus, this organ allows for non-invasive visualization of early changes in CNS disorders. There is a growing interest in developing implantable devices, such as intraocular-lens (IOL), for specific medical uses, including intraocular monitoring. We describe a novel IOL-sensing system for detecting AH biomarkers via biocompatible enzyme-activatable fluorogenic hydrogel sensors. Matrix-metalloproteinase-9, a biomarker of degenerative CNS and eye disorders, was selected as a target. A peptide-probe-incorporated fluorogenic IOL (FIOL) was developed using diacrylamide-group-modified poly(ethyleneglycol) (PEGDAAm) biocompatible hydrogels, adjusting the hydrogel mesh size to allow selective penetration of the target while blocking non-targets, using label-free detection with semi-permanently implantable sensors, and demonstrating the clinical feasibility of FIOL through in vivo testing. This novel FIOL-based sensing system represents a promising approach for liquid biopsy of intraocular fluids.

Original languageEnglish
Article number112254
JournalBiosensors and Bioelectronics
Volume162
DOIs
Publication statusPublished - 2020 Aug 15

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute ( KHIDI ), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C1159 ), and supported by the Nano-Material Technology Development Program and the Basic Science Research Program through the National Research Foundation of Korea ( NRF ), funded by the Ministry of Education, Science and Technology , Republic of Korea (grant number: 2017M3A7B4041798 and 2018R1C1B6002106 , respectively).

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C1159), and supported by the Nano-Material Technology Development Program and the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea (grant number: 2017M3A7B4041798 and 2018R1C1B6002106, respectively).

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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