Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs: 24-week histometric observations

Jung Chul Park, Ulf M.E. Wikesjö, Ki Tae Koo, Jung Seok Lee, Yong Tae Kim, Susanne D. Pippig, Patrizia Bastone, Chang Sung Kim, Chong Kwan Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

Original languageEnglish
Pages (from-to)565-573
Number of pages9
JournalJournal of Clinical Periodontology
Volume39
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

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Polyglactin 910
Dogs
Bone and Bones
Acids
Root Resorption
Ankylosis
Bone Regeneration
Bicuspid
human GDF5 protein
Jaw
Osteogenesis
Mouth
Tooth
Injections

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Park, Jung Chul ; Wikesjö, Ulf M.E. ; Koo, Ki Tae ; Lee, Jung Seok ; Kim, Yong Tae ; Pippig, Susanne D. ; Bastone, Patrizia ; Kim, Chang Sung ; Kim, Chong Kwan. / Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs : 24-week histometric observations. In: Journal of Clinical Periodontology. 2012 ; Vol. 39, No. 6. pp. 565-573.
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abstract = "Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.",
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Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs : 24-week histometric observations. / Park, Jung Chul; Wikesjö, Ulf M.E.; Koo, Ki Tae; Lee, Jung Seok; Kim, Yong Tae; Pippig, Susanne D.; Bastone, Patrizia; Kim, Chang Sung; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 39, No. 6, 01.06.2012, p. 565-573.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs

T2 - 24-week histometric observations

AU - Park, Jung Chul

AU - Wikesjö, Ulf M.E.

AU - Koo, Ki Tae

AU - Lee, Jung Seok

AU - Kim, Yong Tae

AU - Pippig, Susanne D.

AU - Bastone, Patrizia

AU - Kim, Chang Sung

AU - Kim, Chong Kwan

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

AB - Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

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