Mature chief cells are cryptic progenitors for metaplasia in the stomach

Ki Taek Nam, Hyukjoon Lee, Josane F. Sousa, Victoria G. Weis, Ryan L. O'Neal, Paul E. Finke, Judith Romerogallo, Guanglu Shi, Jason C. Mills, Richard M. Peek, Stephen F. Konieczny, James R. Goldenring

Research output: Contribution to journalArticle

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Abstract

Background & Aims Gastric cancer evolves in the setting of a pathologic mucosal milieu characterized by both loss of acid-secreting parietal cells and mucous cell metaplasias. Indeed, mucous cell metaplasia is considered the critical preneoplastic lesion for gastric cancer. Previous investigations have shown that infection of mice with Helicobacter felis or induction of acute parietal cell loss with the drug DMP-777 leads to the emergence of a type of metaplasia designated spasmolytic polypeptide-expressing metaplasia (SPEM). We have hypothesized that SPEM arises from proliferating cells in gland bases, either from a cryptic progenitor cell or by transdifferentiation of mature chief cells. Methods Taking advantage of the chief cell-restricted expression of Mist1-Cre-ERT2, we used lineage mapping to examine whether SPEM lineages were derived from chief cells in 3 independent models of induction by DMP-777 treatment, L-635 treatment, or H felis infection. Results Treatment of mice with L-635 for 3 days led to rapid parietal cell loss, induction of a prominent inflammatory infiltrate, and emergence of SPEM. In all 3 models, SPEM developed, at least in part, from transdifferentiation of chief cells. We further found that acute parietal cell loss in the setting of inflammation (L-635 treatment) led to more rapid induction and expansion of SPEM derived from transdifferentiation of chief cells. Conclusions These studies provide direct evidence by lineage tracing that SPEM evolves from differentiated chief cells. Thus, mature gastric chief cells have the ability to act as cryptic progenitors and reacquire proliferative capacity within the context of mucosal injury and inflammation.

Original languageEnglish
Pages (from-to)2028-2037.e9
JournalGastroenterology
Volume139
Issue number6
DOIs
Publication statusPublished - 2010 Dec

Fingerprint

Metaplasia
Stomach
Stem Cells
Cell Transdifferentiation
DMP 777
Stomach Neoplasms
Gastric Chief Cells
Helicobacter felis
Felis
Inflammation
Infection
spasmolytic polypeptide
Acids

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Nam, K. T., Lee, H., Sousa, J. F., Weis, V. G., O'Neal, R. L., Finke, P. E., ... Goldenring, J. R. (2010). Mature chief cells are cryptic progenitors for metaplasia in the stomach. Gastroenterology, 139(6), 2028-2037.e9. https://doi.org/10.1053/j.gastro.2010.09.005
Nam, Ki Taek ; Lee, Hyukjoon ; Sousa, Josane F. ; Weis, Victoria G. ; O'Neal, Ryan L. ; Finke, Paul E. ; Romerogallo, Judith ; Shi, Guanglu ; Mills, Jason C. ; Peek, Richard M. ; Konieczny, Stephen F. ; Goldenring, James R. / Mature chief cells are cryptic progenitors for metaplasia in the stomach. In: Gastroenterology. 2010 ; Vol. 139, No. 6. pp. 2028-2037.e9.
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abstract = "Background & Aims Gastric cancer evolves in the setting of a pathologic mucosal milieu characterized by both loss of acid-secreting parietal cells and mucous cell metaplasias. Indeed, mucous cell metaplasia is considered the critical preneoplastic lesion for gastric cancer. Previous investigations have shown that infection of mice with Helicobacter felis or induction of acute parietal cell loss with the drug DMP-777 leads to the emergence of a type of metaplasia designated spasmolytic polypeptide-expressing metaplasia (SPEM). We have hypothesized that SPEM arises from proliferating cells in gland bases, either from a cryptic progenitor cell or by transdifferentiation of mature chief cells. Methods Taking advantage of the chief cell-restricted expression of Mist1-Cre-ERT2, we used lineage mapping to examine whether SPEM lineages were derived from chief cells in 3 independent models of induction by DMP-777 treatment, L-635 treatment, or H felis infection. Results Treatment of mice with L-635 for 3 days led to rapid parietal cell loss, induction of a prominent inflammatory infiltrate, and emergence of SPEM. In all 3 models, SPEM developed, at least in part, from transdifferentiation of chief cells. We further found that acute parietal cell loss in the setting of inflammation (L-635 treatment) led to more rapid induction and expansion of SPEM derived from transdifferentiation of chief cells. Conclusions These studies provide direct evidence by lineage tracing that SPEM evolves from differentiated chief cells. Thus, mature gastric chief cells have the ability to act as cryptic progenitors and reacquire proliferative capacity within the context of mucosal injury and inflammation.",
author = "Nam, {Ki Taek} and Hyukjoon Lee and Sousa, {Josane F.} and Weis, {Victoria G.} and O'Neal, {Ryan L.} and Finke, {Paul E.} and Judith Romerogallo and Guanglu Shi and Mills, {Jason C.} and Peek, {Richard M.} and Konieczny, {Stephen F.} and Goldenring, {James R.}",
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Nam, KT, Lee, H, Sousa, JF, Weis, VG, O'Neal, RL, Finke, PE, Romerogallo, J, Shi, G, Mills, JC, Peek, RM, Konieczny, SF & Goldenring, JR 2010, 'Mature chief cells are cryptic progenitors for metaplasia in the stomach', Gastroenterology, vol. 139, no. 6, pp. 2028-2037.e9. https://doi.org/10.1053/j.gastro.2010.09.005

Mature chief cells are cryptic progenitors for metaplasia in the stomach. / Nam, Ki Taek; Lee, Hyukjoon; Sousa, Josane F.; Weis, Victoria G.; O'Neal, Ryan L.; Finke, Paul E.; Romerogallo, Judith; Shi, Guanglu; Mills, Jason C.; Peek, Richard M.; Konieczny, Stephen F.; Goldenring, James R.

In: Gastroenterology, Vol. 139, No. 6, 12.2010, p. 2028-2037.e9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mature chief cells are cryptic progenitors for metaplasia in the stomach

AU - Nam, Ki Taek

AU - Lee, Hyukjoon

AU - Sousa, Josane F.

AU - Weis, Victoria G.

AU - O'Neal, Ryan L.

AU - Finke, Paul E.

AU - Romerogallo, Judith

AU - Shi, Guanglu

AU - Mills, Jason C.

AU - Peek, Richard M.

AU - Konieczny, Stephen F.

AU - Goldenring, James R.

PY - 2010/12

Y1 - 2010/12

N2 - Background & Aims Gastric cancer evolves in the setting of a pathologic mucosal milieu characterized by both loss of acid-secreting parietal cells and mucous cell metaplasias. Indeed, mucous cell metaplasia is considered the critical preneoplastic lesion for gastric cancer. Previous investigations have shown that infection of mice with Helicobacter felis or induction of acute parietal cell loss with the drug DMP-777 leads to the emergence of a type of metaplasia designated spasmolytic polypeptide-expressing metaplasia (SPEM). We have hypothesized that SPEM arises from proliferating cells in gland bases, either from a cryptic progenitor cell or by transdifferentiation of mature chief cells. Methods Taking advantage of the chief cell-restricted expression of Mist1-Cre-ERT2, we used lineage mapping to examine whether SPEM lineages were derived from chief cells in 3 independent models of induction by DMP-777 treatment, L-635 treatment, or H felis infection. Results Treatment of mice with L-635 for 3 days led to rapid parietal cell loss, induction of a prominent inflammatory infiltrate, and emergence of SPEM. In all 3 models, SPEM developed, at least in part, from transdifferentiation of chief cells. We further found that acute parietal cell loss in the setting of inflammation (L-635 treatment) led to more rapid induction and expansion of SPEM derived from transdifferentiation of chief cells. Conclusions These studies provide direct evidence by lineage tracing that SPEM evolves from differentiated chief cells. Thus, mature gastric chief cells have the ability to act as cryptic progenitors and reacquire proliferative capacity within the context of mucosal injury and inflammation.

AB - Background & Aims Gastric cancer evolves in the setting of a pathologic mucosal milieu characterized by both loss of acid-secreting parietal cells and mucous cell metaplasias. Indeed, mucous cell metaplasia is considered the critical preneoplastic lesion for gastric cancer. Previous investigations have shown that infection of mice with Helicobacter felis or induction of acute parietal cell loss with the drug DMP-777 leads to the emergence of a type of metaplasia designated spasmolytic polypeptide-expressing metaplasia (SPEM). We have hypothesized that SPEM arises from proliferating cells in gland bases, either from a cryptic progenitor cell or by transdifferentiation of mature chief cells. Methods Taking advantage of the chief cell-restricted expression of Mist1-Cre-ERT2, we used lineage mapping to examine whether SPEM lineages were derived from chief cells in 3 independent models of induction by DMP-777 treatment, L-635 treatment, or H felis infection. Results Treatment of mice with L-635 for 3 days led to rapid parietal cell loss, induction of a prominent inflammatory infiltrate, and emergence of SPEM. In all 3 models, SPEM developed, at least in part, from transdifferentiation of chief cells. We further found that acute parietal cell loss in the setting of inflammation (L-635 treatment) led to more rapid induction and expansion of SPEM derived from transdifferentiation of chief cells. Conclusions These studies provide direct evidence by lineage tracing that SPEM evolves from differentiated chief cells. Thus, mature gastric chief cells have the ability to act as cryptic progenitors and reacquire proliferative capacity within the context of mucosal injury and inflammation.

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