Measurement of antibodies to varicella-zoster virus using a virus-free fluorescent-antibody-to-membrane-antigen (FAMA) test

Rackhyun Park, Ji Young Hwang, Kang Il Lee, Sim Namkoong, Seuk Keun Choi, Songyong Park, Hosun Park, Junsoo Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The fluorescent-antibody-to-membrane-antigen (FAMA) test is regarded as the “gold standard” to detect protective antibodies to varicella-zoster virus (VZV) because of its high sensitivity and specificity. Because the classic FAMA test uses an infectious virus for detection of antibodies to VZV, it is labor-intensive, and also requires special equipment for handling the virus. For this reason, we attempted to develop a simple and safe FAMA assay. Because VZV glycoprotein E (gE) is one of the major VZV glycoproteins, we used the gE protein for the FAMA test (gE FAMA). Here, we demonstrate that overexpression of gE in HEK293T cells can be used to measure antibodies in human serum, and that gE FAMA titers are closely correlated with gpEIA ELISA data. These results indicate that our gE FAMA test has the potential to measure antibodies to VZV.

Original languageEnglish
Pages (from-to)268-273
Number of pages6
JournalJournal of microbiology and biotechnology
Volume25
Issue number2
DOIs
Publication statusPublished - 2015 Feb 28

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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