TY - JOUR
T1 - Measurement of cellulase and xylosidase activities in peat using a sensitive fluorogenic compound assay
AU - Kang, H.
AU - Freeman, C.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - We applied fluorogenic methylumbelliferyl compounds to measure enzyme activities (β-glucosidase, cellobiohydrolase, and β-xylosidase) in a wetland peat. The enzyme activities showed substrate saturation around 400 μM and approximate linearity for at least 100 minutes incubation time. The effects of phenolics and toluene on the enzymes were small. All enzyme activities increased with increasing incubation temperature. Beta-glucosidase and β-xylosidase showed optimal activities in an acidic condition (pH 5), while cellobiohydrolase had the highest activity in a neutral pH. The method would be effective in samples with low enzyme activities and interferences from organic compounds.
AB - We applied fluorogenic methylumbelliferyl compounds to measure enzyme activities (β-glucosidase, cellobiohydrolase, and β-xylosidase) in a wetland peat. The enzyme activities showed substrate saturation around 400 μM and approximate linearity for at least 100 minutes incubation time. The effects of phenolics and toluene on the enzymes were small. All enzyme activities increased with increasing incubation temperature. Beta-glucosidase and β-xylosidase showed optimal activities in an acidic condition (pH 5), while cellobiohydrolase had the highest activity in a neutral pH. The method would be effective in samples with low enzyme activities and interferences from organic compounds.
UR - http://www.scopus.com/inward/record.url?scp=0031663418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031663418&partnerID=8YFLogxK
U2 - 10.1080/00103629809370151
DO - 10.1080/00103629809370151
M3 - Article
AN - SCOPUS:0031663418
VL - 29
SP - 2769
EP - 2774
JO - Communications in Soil Science and Plant Analysis
JF - Communications in Soil Science and Plant Analysis
SN - 0010-3624
IS - 17-18
ER -