We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
|Number of pages||7|
|Journal||Archiv fur Hydrobiologie|
|Publication status||Published - 1997|
All Science Journal Classification (ASJC) codes
- Aquatic Science