TY - JOUR
T1 - Measurement of phosphomonoesterase activity in wetland sediments - A sensitive method using HPLC and UV detection
AU - Kang, Hojeong
AU - Freeman, Chris
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
AB - We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
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U2 - 10.1127/archiv-hydrobiol/140/1997/411
DO - 10.1127/archiv-hydrobiol/140/1997/411
M3 - Article
AN - SCOPUS:0030827645
VL - 140
SP - 411
EP - 417
JO - Fundamental and Applied Limnology
JF - Fundamental and Applied Limnology
SN - 1863-9135
IS - 3
ER -