Measurement of phosphomonoesterase activity in wetland sediments - A sensitive method using HPLC and UV detection

Hojeong Kang, Chris Freeman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.

Original languageEnglish
Pages (from-to)411-417
Number of pages7
JournalArchiv fur Hydrobiologie
Volume140
Issue number3
Publication statusPublished - 1997 Jan 1

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wetlands
high performance liquid chromatography
wetland
substrate
sediments
assays
assay
sediment
detectors
linearity
incubation
methodology
saturation
matrix
sampling
detection
method
detector
product

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

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Measurement of phosphomonoesterase activity in wetland sediments - A sensitive method using HPLC and UV detection. / Kang, Hojeong; Freeman, Chris.

In: Archiv fur Hydrobiologie, Vol. 140, No. 3, 01.01.1997, p. 411-417.

Research output: Contribution to journalArticle

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