Abstract
We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
| Original language | English |
|---|---|
| Pages (from-to) | 411-417 |
| Number of pages | 7 |
| Journal | Archiv fur Hydrobiologie |
| Volume | 140 |
| Issue number | 3 |
| Publication status | Published - 1997 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Aquatic Science
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Measurement of phosphomonoesterase activity in wetland sediments - A sensitive method using HPLC and UV detection. / Kang, Hojeong; Freeman, Chris.
In: Archiv fur Hydrobiologie, Vol. 140, No. 3, 01.01.1997, p. 411-417.Research output: Contribution to journal › Article
TY - JOUR
T1 - Measurement of phosphomonoesterase activity in wetland sediments - A sensitive method using HPLC and UV detection
AU - Kang, Hojeong
AU - Freeman, Chris
PY - 1997/1/1
Y1 - 1997/1/1
N2 - We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
AB - We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800μM of substrate concentration.
UR - http://www.scopus.com/inward/record.url?scp=0030827645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030827645&partnerID=8YFLogxK
M3 - Article
VL - 140
SP - 411
EP - 417
JO - Fundamental and Applied Limnology
JF - Fundamental and Applied Limnology
SN - 1863-9135
IS - 3
ER -