Measurement of relative phase distribution of onion epidermal cells by using the polarization microscope

In Hee Shin, Ji Yong Lee, Seungrag Lee, Dong Ju Lee, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Bio-cells and tissues have intrinsic polarization characteristics, which are changed by external stimulus and internal metamorphosis in cells and tissues and some of the bio-cells and tissues have intrinsic birefringence characteristics, which are also changed by external stimulus and internal metamorphosis in cells and tissues. In this paper, we have developed the polarization microscope for measurement of relative phase which results from birefringence characteristics of materials with improved linear polarizing method and have measured relative phase distribution of onion epidermal cells. From the measurement of the relative phase distribution of onion epidermal cells, decrease of relative phase distribution of onion epidermal cells was investigated as the elapse of time. In decrease of relative phase distribution, relative phase of cell membrane in onion epidermal cells decreased radically as compared with that of cytoplasm because decline of function in cell membrane that takes charge of matter transfer in onion epidermal cells has occurred.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XIV
DOIs
Publication statusPublished - 2007 Apr 30
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV - San Jose, CA, United States
Duration: 2007 Jan 232007 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6443
ISSN (Print)1605-7422

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
CountryUnited States
CitySan Jose, CA
Period07/1/2307/1/25

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Shin, I. H., Lee, J. Y., Lee, S., Lee, D. J., & Kim, D. Y. (2007). Measurement of relative phase distribution of onion epidermal cells by using the polarization microscope. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV [644317] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6443). https://doi.org/10.1117/12.699785