Measuring phosphatase activity in peatland soils

Recent methodological advances

Chris Freeman, Inyoung Jang, Kyoung Duk Zho, Hojeong Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalEnvironmental Engineering Research
Volume16
Issue number4
Publication statusPublished - 2011 Dec 1

Fingerprint

Phosphatases
Biological materials
Assays
Sediments
Soils
Peat
Wetlands
Quenching
Hydrolysis

All Science Journal Classification (ASJC) codes

  • Environmental Engineering

Cite this

Freeman, Chris ; Jang, Inyoung ; Zho, Kyoung Duk ; Kang, Hojeong. / Measuring phosphatase activity in peatland soils : Recent methodological advances. In: Environmental Engineering Research. 2011 ; Vol. 16, No. 4. pp. 165-168.
@article{c2cebb3b12224801a3d929ff47ddc934,
title = "Measuring phosphatase activity in peatland soils: Recent methodological advances",
abstract = "Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.",
author = "Chris Freeman and Inyoung Jang and Zho, {Kyoung Duk} and Hojeong Kang",
year = "2011",
month = "12",
day = "1",
language = "English",
volume = "16",
pages = "165--168",
journal = "Environmental Engineering Research",
issn = "1226-1025",
publisher = "Korean Society of Environmental Engineers",
number = "4",

}

Measuring phosphatase activity in peatland soils : Recent methodological advances. / Freeman, Chris; Jang, Inyoung; Zho, Kyoung Duk; Kang, Hojeong.

In: Environmental Engineering Research, Vol. 16, No. 4, 01.12.2011, p. 165-168.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Measuring phosphatase activity in peatland soils

T2 - Recent methodological advances

AU - Freeman, Chris

AU - Jang, Inyoung

AU - Zho, Kyoung Duk

AU - Kang, Hojeong

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.

AB - Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.

UR - http://www.scopus.com/inward/record.url?scp=84856559863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856559863&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 165

EP - 168

JO - Environmental Engineering Research

JF - Environmental Engineering Research

SN - 1226-1025

IS - 4

ER -