Measuring phosphatase activity in peatland soils: Recent methodological advances

Chris Freeman, Inyoung Jang, Kyoung Duk Zho, Hojeong Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalEnvironmental Engineering Research
Volume16
Issue number4
Publication statusPublished - 2011 Dec 1

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All Science Journal Classification (ASJC) codes

  • Environmental Engineering

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