This paper firstly reports the mechanical and real-time detection of liposomes using the highly sensitive microfabricated piezoresistive cantilever sensor chip and liposome-protein interaction. The cantilevers were functionalized with the chemically modified protein C2A which recognizes the phosphatidylserine (PS) exposed on the surface of liposome. The attachment of liposome induced the bending motion of the piezoresistive cantilever and consequently generated the electrical signal changes. The sensitivity of the sensor was determined from the relationship between electrical signal and the number of liposome immobilized on the cantilever surface. This result will help challenge a new detection method to monitor various micro-sized organisms such as bacteria and animal cells.
Bibliographical noteFunding Information:
This work was supported by a grant from National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation. This work was made possible in part through the use of research facilities in Yonsei Center for Biotechnology. We would like to thank professor Kyung-Hwa Yoo for helpful discussion and Mr. Hyun-Seok Lee for technical supports. We are also grateful to professor B. Davletov for his kind supply of C2A protein gene.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry