Abstract
α-Lipoic acid (LA) shows a protective effect on oxidative stress-induced apoptosis while it induces apoptosis in various cancer cells. Intracellular Ca2+ plays a central role in triggering apoptotic pathways. In the present study, we aim to investigate whether LA induces apoptosis in lung cancer cells and whether Ca2+ is involved in LA-induced apoptosis. We found that LA decreased cell viability and increased DNA fragmentation of the cells. LA activated the caspase-independent pathway, determined by upregulation of poly(ADP-ribose) polymerase (PARP) and increased the nuclear level of apoptosis-inducing factor and caspase-dependent apoptotic pathway, determined by increased levels of cytochrome c and PARP-1 cleavage product. LA-induced apoptotic alterations were inhibited in the cells treated with Ca2+ chelator BAPTA-AM. In conclusion, LA induces apoptosis through caspase-independent and caspase-dependent pathways, which is mediated by intracellular Ca2+.
| Original language | English |
|---|---|
| Title of host publication | Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways |
| Publisher | Blackwell Publishing Inc. |
| Pages | 149-155 |
| Number of pages | 7 |
| ISBN (Print) | 9781573317375 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
Publication series
| Name | Annals of the New York Academy of Sciences |
|---|---|
| Volume | 1171 |
| ISSN (Print) | 0077-8923 |
| ISSN (Electronic) | 1749-6632 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science
Cite this
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Mechanism of α-lipoic acid-induced apoptosis of lung cancer cells : Involvement of Ca2+. / Choi, Sang Yong; Yu, Ji Hoon; Kim, Hye Young.
Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. p. 149-155 (Annals of the New York Academy of Sciences; Vol. 1171).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Mechanism of α-lipoic acid-induced apoptosis of lung cancer cells
T2 - Involvement of Ca2+
AU - Choi, Sang Yong
AU - Yu, Ji Hoon
AU - Kim, Hye Young
PY - 2009/1/1
Y1 - 2009/1/1
N2 - α-Lipoic acid (LA) shows a protective effect on oxidative stress-induced apoptosis while it induces apoptosis in various cancer cells. Intracellular Ca2+ plays a central role in triggering apoptotic pathways. In the present study, we aim to investigate whether LA induces apoptosis in lung cancer cells and whether Ca2+ is involved in LA-induced apoptosis. We found that LA decreased cell viability and increased DNA fragmentation of the cells. LA activated the caspase-independent pathway, determined by upregulation of poly(ADP-ribose) polymerase (PARP) and increased the nuclear level of apoptosis-inducing factor and caspase-dependent apoptotic pathway, determined by increased levels of cytochrome c and PARP-1 cleavage product. LA-induced apoptotic alterations were inhibited in the cells treated with Ca2+ chelator BAPTA-AM. In conclusion, LA induces apoptosis through caspase-independent and caspase-dependent pathways, which is mediated by intracellular Ca2+.
AB - α-Lipoic acid (LA) shows a protective effect on oxidative stress-induced apoptosis while it induces apoptosis in various cancer cells. Intracellular Ca2+ plays a central role in triggering apoptotic pathways. In the present study, we aim to investigate whether LA induces apoptosis in lung cancer cells and whether Ca2+ is involved in LA-induced apoptosis. We found that LA decreased cell viability and increased DNA fragmentation of the cells. LA activated the caspase-independent pathway, determined by upregulation of poly(ADP-ribose) polymerase (PARP) and increased the nuclear level of apoptosis-inducing factor and caspase-dependent apoptotic pathway, determined by increased levels of cytochrome c and PARP-1 cleavage product. LA-induced apoptotic alterations were inhibited in the cells treated with Ca2+ chelator BAPTA-AM. In conclusion, LA induces apoptosis through caspase-independent and caspase-dependent pathways, which is mediated by intracellular Ca2+.
UR - http://www.scopus.com/inward/record.url?scp=69149086638&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69149086638&partnerID=8YFLogxK
U2 - 10.1111/j.1749-6632.2009.04708.x
DO - 10.1111/j.1749-6632.2009.04708.x
M3 - Conference contribution
SN - 9781573317375
T3 - Annals of the New York Academy of Sciences
SP - 149
EP - 155
BT - Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PB - Blackwell Publishing Inc.
ER -