TY - GEN
T1 - Mechanism of α-lipoic acid-induced apoptosis of lung cancer cells
T2 - Involvement of Ca2+
AU - Choi, Sang Yong
AU - Yu, Ji Hoon
AU - Kim, Hyeyoung
PY - 2009/8
Y1 - 2009/8
N2 - α-Lipoic acid (LA) shows a protective effect on oxidative stress-induced apoptosis while it induces apoptosis in various cancer cells. Intracellular Ca2+ plays a central role in triggering apoptotic pathways. In the present study, we aim to investigate whether LA induces apoptosis in lung cancer cells and whether Ca2+ is involved in LA-induced apoptosis. We found that LA decreased cell viability and increased DNA fragmentation of the cells. LA activated the caspase-independent pathway, determined by upregulation of poly(ADP-ribose) polymerase (PARP) and increased the nuclear level of apoptosis-inducing factor and caspase-dependent apoptotic pathway, determined by increased levels of cytochrome c and PARP-1 cleavage product. LA-induced apoptotic alterations were inhibited in the cells treated with Ca2+ chelator BAPTA-AM. In conclusion, LA induces apoptosis through caspase-independent and caspase-dependent pathways, which is mediated by intracellular Ca2+.
AB - α-Lipoic acid (LA) shows a protective effect on oxidative stress-induced apoptosis while it induces apoptosis in various cancer cells. Intracellular Ca2+ plays a central role in triggering apoptotic pathways. In the present study, we aim to investigate whether LA induces apoptosis in lung cancer cells and whether Ca2+ is involved in LA-induced apoptosis. We found that LA decreased cell viability and increased DNA fragmentation of the cells. LA activated the caspase-independent pathway, determined by upregulation of poly(ADP-ribose) polymerase (PARP) and increased the nuclear level of apoptosis-inducing factor and caspase-dependent apoptotic pathway, determined by increased levels of cytochrome c and PARP-1 cleavage product. LA-induced apoptotic alterations were inhibited in the cells treated with Ca2+ chelator BAPTA-AM. In conclusion, LA induces apoptosis through caspase-independent and caspase-dependent pathways, which is mediated by intracellular Ca2+.
UR - http://www.scopus.com/inward/record.url?scp=69149086638&partnerID=8YFLogxK
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U2 - 10.1111/j.1749-6632.2009.04708.x
DO - 10.1111/j.1749-6632.2009.04708.x
M3 - Conference contribution
C2 - 19723049
AN - SCOPUS:69149086638
SN - 9781573317375
T3 - Annals of the New York Academy of Sciences
SP - 149
EP - 155
BT - Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PB - Blackwell Publishing Inc.
ER -