Mechanism of β-carotene-induced apoptosis of gastric cancer cells: Involvement of ataxia-telangiectasia-mutated

Sung Hee Jang, Joo Weon Lim, Hyeyoung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Citations (Scopus)

Abstract

β-Carotene acts as an antioxidant or a pro-oxidant depending on the concentrations that cells are treated with. Oxidative DNA damage is related to apoptosis of various cells. Ataxia-telangiectasia-mutated (ATM), a sensor for DNA-damaging agents, activates a variety of effectors in multiple signaling pathways, such as DNA repair and apoptosis. In the present study, we investigated whether a high concentration of β-carotene induces apoptosis of gastric adenocarcinoma (AGS) cells and whether ATM is involved in β-carotene-induced apoptosis of AGS cells. We found that β-carotene (100 μmol/L) induced apoptosis (determined by cell viability), DNA fragmentation, and the protein levels of p53 and Bcl-2 in AGS cells. ATM levels in the nucleus decreased from β-carotene in AGS cells. β-Carotene- induced alterations, including an increase in DNA fragmentation and p53 levels and a decrease in nuclear ATM and cellular Bcl-2 levels, were inhibited in the cells transfected with full-length ATM cDNA compared to wild-type cells or the cells transfected with control vector plasmid control DNA vector (pcDNA). In conclusion, β-carotene induces apoptosis by increasing apoptotic protein p53 and decreasing anti-apoptotic Bcl-2 as well as nuclear ATM in AGS cells. Nuclear loss of ATM may be the underlying mechanism of β-carotene-induced apoptosis of gastric cancer cells.

Original languageEnglish
Title of host publicationNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PublisherBlackwell Publishing Inc.
Pages156-162
Number of pages7
ISBN (Print)9781573317375
DOIs
Publication statusPublished - 2009 Aug

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Jang, S. H., Lim, J. W., & Kim, H. (2009). Mechanism of β-carotene-induced apoptosis of gastric cancer cells: Involvement of ataxia-telangiectasia-mutated. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (pp. 156-162). (Annals of the New York Academy of Sciences; Vol. 1171). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04711.x