MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators

Tae Whan Kim, Yong Jae Kwon, Jung Mo Kim, Young Hwa Song, Se Nyun Kim, Young-Joon Kim

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Transcriptional activators interact with diverse proteins and recruit transcriptional machinery to the activated promoter. Recruitment of the Mediator complex by transcriptional activators is usually the key step in transcriptional activation. However, it is unclear how Mediator recognizes different types of activator proteins. To systematically identify the subunits responsible for the signal- and activator-specific functions of Mediator in Drosophila melanogaster, each Mediator subunit was depleted by RNA interference, and its effect on transcriptional activation of endogenous as well as synthetic promoters was examined. The depletion of some Mediator gene products caused general transcriptional defects, whereas depletion of others caused defects specifically related to activation. In particular, MED16 and MED23 were required for lipopolysaccharide- and heat-shock-specific gene expression, respectively, and their activator-specific functions appeared to result from interaction with specific activators. The corequirement of MED16 for other forms of differentiation-inducing factor-induced transcription was confirmed by microarray analysis of differentiation-inducing factor (DIF)- and MED16-depleted cells individually. These results suggest that distinct Mediator subunits interact with specific activators to coordinate and transfer activator-specific signals to the transcriptional machinery.

Original languageEnglish
Pages (from-to)12153-12158
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number33
DOIs
Publication statusPublished - 2004 Aug 17

Fingerprint

Activator Appliances
Transcriptional Activation
Lipopolysaccharides
Shock
Hot Temperature
Mediator Complex
Microarray Analysis
RNA Interference
Drosophila melanogaster
Proteins
Gene Expression
Genes
1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{f11f1f750c064a679b716f7051af9f98,
title = "MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators",
abstract = "Transcriptional activators interact with diverse proteins and recruit transcriptional machinery to the activated promoter. Recruitment of the Mediator complex by transcriptional activators is usually the key step in transcriptional activation. However, it is unclear how Mediator recognizes different types of activator proteins. To systematically identify the subunits responsible for the signal- and activator-specific functions of Mediator in Drosophila melanogaster, each Mediator subunit was depleted by RNA interference, and its effect on transcriptional activation of endogenous as well as synthetic promoters was examined. The depletion of some Mediator gene products caused general transcriptional defects, whereas depletion of others caused defects specifically related to activation. In particular, MED16 and MED23 were required for lipopolysaccharide- and heat-shock-specific gene expression, respectively, and their activator-specific functions appeared to result from interaction with specific activators. The corequirement of MED16 for other forms of differentiation-inducing factor-induced transcription was confirmed by microarray analysis of differentiation-inducing factor (DIF)- and MED16-depleted cells individually. These results suggest that distinct Mediator subunits interact with specific activators to coordinate and transfer activator-specific signals to the transcriptional machinery.",
author = "Kim, {Tae Whan} and Kwon, {Yong Jae} and Kim, {Jung Mo} and Song, {Young Hwa} and Kim, {Se Nyun} and Young-Joon Kim",
year = "2004",
month = "8",
day = "17",
doi = "10.1073/pnas.0401985101",
language = "English",
volume = "101",
pages = "12153--12158",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "33",

}

MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators. / Kim, Tae Whan; Kwon, Yong Jae; Kim, Jung Mo; Song, Young Hwa; Kim, Se Nyun; Kim, Young-Joon.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 33, 17.08.2004, p. 12153-12158.

Research output: Contribution to journalArticle

TY - JOUR

T1 - MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators

AU - Kim, Tae Whan

AU - Kwon, Yong Jae

AU - Kim, Jung Mo

AU - Song, Young Hwa

AU - Kim, Se Nyun

AU - Kim, Young-Joon

PY - 2004/8/17

Y1 - 2004/8/17

N2 - Transcriptional activators interact with diverse proteins and recruit transcriptional machinery to the activated promoter. Recruitment of the Mediator complex by transcriptional activators is usually the key step in transcriptional activation. However, it is unclear how Mediator recognizes different types of activator proteins. To systematically identify the subunits responsible for the signal- and activator-specific functions of Mediator in Drosophila melanogaster, each Mediator subunit was depleted by RNA interference, and its effect on transcriptional activation of endogenous as well as synthetic promoters was examined. The depletion of some Mediator gene products caused general transcriptional defects, whereas depletion of others caused defects specifically related to activation. In particular, MED16 and MED23 were required for lipopolysaccharide- and heat-shock-specific gene expression, respectively, and their activator-specific functions appeared to result from interaction with specific activators. The corequirement of MED16 for other forms of differentiation-inducing factor-induced transcription was confirmed by microarray analysis of differentiation-inducing factor (DIF)- and MED16-depleted cells individually. These results suggest that distinct Mediator subunits interact with specific activators to coordinate and transfer activator-specific signals to the transcriptional machinery.

AB - Transcriptional activators interact with diverse proteins and recruit transcriptional machinery to the activated promoter. Recruitment of the Mediator complex by transcriptional activators is usually the key step in transcriptional activation. However, it is unclear how Mediator recognizes different types of activator proteins. To systematically identify the subunits responsible for the signal- and activator-specific functions of Mediator in Drosophila melanogaster, each Mediator subunit was depleted by RNA interference, and its effect on transcriptional activation of endogenous as well as synthetic promoters was examined. The depletion of some Mediator gene products caused general transcriptional defects, whereas depletion of others caused defects specifically related to activation. In particular, MED16 and MED23 were required for lipopolysaccharide- and heat-shock-specific gene expression, respectively, and their activator-specific functions appeared to result from interaction with specific activators. The corequirement of MED16 for other forms of differentiation-inducing factor-induced transcription was confirmed by microarray analysis of differentiation-inducing factor (DIF)- and MED16-depleted cells individually. These results suggest that distinct Mediator subunits interact with specific activators to coordinate and transfer activator-specific signals to the transcriptional machinery.

UR - http://www.scopus.com/inward/record.url?scp=4344601432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344601432&partnerID=8YFLogxK

U2 - 10.1073/pnas.0401985101

DO - 10.1073/pnas.0401985101

M3 - Article

VL - 101

SP - 12153

EP - 12158

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 33

ER -