Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock

Jin Mo Park; Janis Werner; Jung Mo Kim; John T. Lis; Young Joon Kim

doi:10.1016/S1097-2765(01)00296-9

Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock

Jin Mo Park, Janis Werner, Jung Mo Kim, John T. Lis, Young Joon Kim

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro. Whether such interactions play a primary regulatory role in recruiting these factors to activator-associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites. Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery.

Original language	English
Pages (from-to)	9-19
Number of pages	11
Journal	Molecular Cell
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/S1097-2765(01)00296-9
Publication status	Published - 2001

Bibliographical note

Funding Information:
We thank Carl Wu, Michael Koelle, Shin-ichi Yanagawa, and Deborah Johnson for plasmids; Bob Tjian and Erik Andrulis for generously providing the anti-TAF250 and anti-CTD antibodies; and Tomohisa Kato for helpful advice. This work was supported by the Creative Research Initiatives Program from the Korean Ministry of Science and Technology to Y.-J.K. and by NIH grant GM25232 to J.T.L.

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1016/S1097-2765(01)00296-9

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title = "Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock",

abstract = "Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro. Whether such interactions play a primary regulatory role in recruiting these factors to activator-associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites. Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery.",

author = "Park, {Jin Mo} and Janis Werner and Kim, {Jung Mo} and Lis, {John T.} and Kim, {Young Joon}",

note = "Funding Information: We thank Carl Wu, Michael Koelle, Shin-ichi Yanagawa, and Deborah Johnson for plasmids; Bob Tjian and Erik Andrulis for generously providing the anti-TAF250 and anti-CTD antibodies; and Tomohisa Kato for helpful advice. This work was supported by the Creative Research Initiatives Program from the Korean Ministry of Science and Technology to Y.-J.K. and by NIH grant GM25232 to J.T.L.",

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T1 - Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock

AU - Park, Jin Mo

AU - Werner, Janis

AU - Kim, Jung Mo

AU - Lis, John T.

AU - Kim, Young Joon

N1 - Funding Information: We thank Carl Wu, Michael Koelle, Shin-ichi Yanagawa, and Deborah Johnson for plasmids; Bob Tjian and Erik Andrulis for generously providing the anti-TAF250 and anti-CTD antibodies; and Tomohisa Kato for helpful advice. This work was supported by the Creative Research Initiatives Program from the Korean Ministry of Science and Technology to Y.-J.K. and by NIH grant GM25232 to J.T.L.

PY - 2001

Y1 - 2001

N2 - Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro. Whether such interactions play a primary regulatory role in recruiting these factors to activator-associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites. Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery.

AB - Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro. Whether such interactions play a primary regulatory role in recruiting these factors to activator-associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites. Unlike the TAFs and Pol II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery.

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JO - Molecular Cell

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Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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