Mediator of transcriptional regulation

Stefan Björklund, Young-Joon Kim

Research output: Contribution to journalReview article

103 Citations (Scopus)

Abstract

A multi-protein complex, termed mediator, has been isolated from yeast, based on its requirement for transcriptional activation in a system reconstituted from pure RNA polymerase II and general transcription factors. Mediator polypeptides include the products of many genes previously recovered from screens for mutations affecting transcription. This connection between biochemical and genetic studies reveals that mediator is important for both activation and repression of transcription, and that mediator plays a role in transcriptional regulation in vivo as well as in vitro. Mediator binds the carboxy-terminal domain of RNA polymerase II, forming a polymerase holoenzyme, whose possible association with additional proteins is a subject of some controversy.

Original languageEnglish
Pages (from-to)335-337
Number of pages3
JournalTrends in Biochemical Sciences
Volume21
Issue number9
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

RNA Polymerase II
Transcription
Transcriptional Activation
Mediator Complex
Chemical activation
General Transcription Factors
Holoenzymes
Yeast
Molecular Biology
Proteins
Genes
Yeasts
Association reactions
Peptides
Mutation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Björklund, Stefan ; Kim, Young-Joon. / Mediator of transcriptional regulation. In: Trends in Biochemical Sciences. 1996 ; Vol. 21, No. 9. pp. 335-337.
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Mediator of transcriptional regulation. / Björklund, Stefan; Kim, Young-Joon.

In: Trends in Biochemical Sciences, Vol. 21, No. 9, 01.01.1996, p. 335-337.

Research output: Contribution to journalReview article

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