Mel-18 is a mammalian homolog of Polycomb group (PcG) genes. Microarray analysis revealed that Mel-18 expression was induced during endothelial progenitor cell (EPC) differentiation and correlates with the expression of EC-specific protein markers. Overexpression of Mel-18 promoted EPC differentiation and angiogenic activity of ECs. Accordingly, silencing Mel-18 inhibited EC migration and tube formation in vitro. Gene expression profiling showed that Mel-18 regulates angiogenic genes including kinase insert domain receptor (KDR), claudin 5, and angiopoietin-like 2. Our findings demonstrate, for the first time, that Mel-18 plays a significant role in the angiogenic function of ECs by regulating endothelial gene expression.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2010 Oct 1|
Bibliographical noteFunding Information:
This work was supported by a National Research Laboratory grant ( 20100018854 ) and the Korea Biotech R&D Group of Next-generation growth engine project ( 2010K0012733-08A0301-00310 ) from the Ministry of Education and Science Technology, and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs ( A085136 ), Korea.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology