Membrane fixation enhances guided bone regeneration in standardized calvarial defects: A pre-clinical study

Yin Zhe An, Franz Josef Strauss, Jin Young Park, Yu Qin Shen, Daniel Stefan Thoma, Jung Seok Lee

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Aim: To determine whether collagen membrane (CM) fixation enhances guided bone regeneration in standardized defects. Materials and Methods: Four 8-mm-diameter defects were surgically made in eight rabbit calvaria, and randomly allocated into four groups: control (empty), unfixed-CM, fixed-CM, and unfixed-CM with bone graft (BG + CM) (positive control). After 1- and 4-week healing periods, the animals were sacrificed and quantitative reverse transcription polymerase chain reaction, micro-computed tomography, and histological outcomes were assessed. Results: At week 1, the expression levels of BMP-2, FGF-2, VEGF, and osteocalcin were significantly higher in the fixed-CM group than in the unfixed-CM and control groups (p <.05). Conversely, cathepsin-K was significantly expressed in the unfixed-CM group. No significant differences in expression markers were observed between the fixed-CM and BG + CM groups (p >.05). At week 4, new bone formation was significantly higher in the fixed-CM group than the unfixed-CM and control groups (p <.05), but similar to the BG + CM group (p >.05). Conclusions: CM fixation enhances the expression of osteogenic factors similar to BG + CM, leading to significantly more new bone formation. This suggests that the osteogenic potential is greater when membranes are fixed, thereby limiting the necessity of membrane-supporting materials to enhance bone formation.

Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalJournal of Clinical Periodontology
Volume49
Issue number2
DOIs
Publication statusPublished - 2022 Feb

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (KMDF_PR_20200901_0238).

Publisher Copyright:
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

All Science Journal Classification (ASJC) codes

  • Periodontics

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