Membrane-reinforced three-dimensional electrospun silk fibroin scaffolds for bone tissue engineering

Sung Yeun Yang, Tae Heon Hwang, Lihua Che, Jin Soo Oh, Yoon Ha, Wonhyoung Ryu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Electrospun silk fibroin (SF) scaffolds have drawn much attention because of their resemblance to natural tissue architecture such as extracellular matrix, and the biocompatibility of SF as a candidate material to replace collagen. However, electrospun scaffolds lack the physical integrity of bone tissue scaffolds, which require resistance to mechanical loadings. In this work, we propose membrane-reinforced electrospun SF scaffolds by a serial process of electrospinning and freeze-drying of SF solutions in two different solvents: formic acid and water, respectively. After wet electrospinning followed by replacement of methanol with water, SF nanofibers dispersed in water were mixed with aqueous SF solution. Freeze-drying of the mixed solution resulted in 3D membrane-connected SF nanofibrous scaffolds (SF scaffolds) with a thickness of a few centimeters. We demonstrated that the SF concentration of aqueous SF solution controlled the degree of membrane reinforcement between nanofibers. It was also shown that both increase in degree of membrane reinforcement and inclusion of hydroxyapatite (HAP) nanoparticles resulted in higher resistance to compressive loadings of the SF scaffolds. Culture of human osteoblasts on collagen, SF, and SF-HAP scaffolds showed that both SF and SF-HAP scaffolds had biocompatibility and cell proliferation superior to that of the collagen scaffolds. SF-HAP scaffolds with and without BMP-2 were used for in vivo studies for 4 and 8 weeks, and they showed enhanced bone tissue formation in rat calvarial defect models.

Original languageEnglish
Article number035011
JournalBiomedical Materials (Bristol)
Volume10
Issue number3
DOIs
Publication statusPublished - 2015 Jun 1

Fingerprint

Fibroins
Silk
Scaffolds (biology)
Tissue engineering
Bone
Membranes
Scaffolds
Durapatite
Hydroxyapatite
Collagen
formic acid
Electrospinning
Nanofibers
Biocompatibility
Water
Drying
Reinforcement
Tissue
Tissue Scaffolds
Formic acid

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "Electrospun silk fibroin (SF) scaffolds have drawn much attention because of their resemblance to natural tissue architecture such as extracellular matrix, and the biocompatibility of SF as a candidate material to replace collagen. However, electrospun scaffolds lack the physical integrity of bone tissue scaffolds, which require resistance to mechanical loadings. In this work, we propose membrane-reinforced electrospun SF scaffolds by a serial process of electrospinning and freeze-drying of SF solutions in two different solvents: formic acid and water, respectively. After wet electrospinning followed by replacement of methanol with water, SF nanofibers dispersed in water were mixed with aqueous SF solution. Freeze-drying of the mixed solution resulted in 3D membrane-connected SF nanofibrous scaffolds (SF scaffolds) with a thickness of a few centimeters. We demonstrated that the SF concentration of aqueous SF solution controlled the degree of membrane reinforcement between nanofibers. It was also shown that both increase in degree of membrane reinforcement and inclusion of hydroxyapatite (HAP) nanoparticles resulted in higher resistance to compressive loadings of the SF scaffolds. Culture of human osteoblasts on collagen, SF, and SF-HAP scaffolds showed that both SF and SF-HAP scaffolds had biocompatibility and cell proliferation superior to that of the collagen scaffolds. SF-HAP scaffolds with and without BMP-2 were used for in vivo studies for 4 and 8 weeks, and they showed enhanced bone tissue formation in rat calvarial defect models.",
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Membrane-reinforced three-dimensional electrospun silk fibroin scaffolds for bone tissue engineering. / Yang, Sung Yeun; Hwang, Tae Heon; Che, Lihua; Oh, Jin Soo; Ha, Yoon; Ryu, Wonhyoung.

In: Biomedical Materials (Bristol), Vol. 10, No. 3, 035011, 01.06.2015.

Research output: Contribution to journalArticle

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AU - Hwang, Tae Heon

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AU - Ha, Yoon

AU - Ryu, Wonhyoung

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