Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells

Chang Hoon Kim, Sung Shik Kim, Jae Young Choi, Ji Hyun Shin, Jin Young Kim, Wan Namkung, Jeung Gweon Lee, Min Goo Lee, Joo Heon Yoon

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency, and mucin secretion. Therefore, this study aims to investigate the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells, In RT-PCR, the mRNAs for several P2X (P2X3, P2X 4, P2X7) and P2Y (P2Y1, P2Y2, (P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring intracellular calcium concentration ([Ca 2+]i) increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of αβ-methylene ATP and 2-methylthio-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase is concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies. In addition, the results with 2,3-O-(4-benzoyl)- benzoyl-ATP indicate that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y 2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.

Original languageEnglish
Pages (from-to)L835-L842
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number4 31-4
DOIs
Publication statusPublished - 2004 Oct 1

Fingerprint

Purinergic Receptors
Nose
Purinergic P2Y2 Receptors
Epithelial Cells
Membranes
Mucins
Purinergic P2X4 Receptors
Purinergic P2X3 Receptors
Adenosine Triphosphate
Purinergic P2Y1 Receptors
Fluids and Secretions
Pyrimidines
Purines
Uridine Triphosphate
Ion Transport
Cell Surface Receptors
Immunoblotting
Perfusion
Calcium
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Kim, Chang Hoon ; Kim, Sung Shik ; Choi, Jae Young ; Shin, Ji Hyun ; Kim, Jin Young ; Namkung, Wan ; Lee, Jeung Gweon ; Lee, Min Goo ; Yoon, Joo Heon. / Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; Vol. 287, No. 4 31-4. pp. L835-L842.
@article{190314ad63e147648d937ca2de10f9e3,
title = "Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells",
abstract = "Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency, and mucin secretion. Therefore, this study aims to investigate the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells, In RT-PCR, the mRNAs for several P2X (P2X3, P2X 4, P2X7) and P2Y (P2Y1, P2Y2, (P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring intracellular calcium concentration ([Ca 2+]i) increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of αβ-methylene ATP and 2-methylthio-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase is concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies. In addition, the results with 2,3-O-(4-benzoyl)- benzoyl-ATP indicate that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y 2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.",
author = "Kim, {Chang Hoon} and Kim, {Sung Shik} and Choi, {Jae Young} and Shin, {Ji Hyun} and Kim, {Jin Young} and Wan Namkung and Lee, {Jeung Gweon} and Lee, {Min Goo} and Yoon, {Joo Heon}",
year = "2004",
month = "10",
day = "1",
doi = "10.1152/ajplung.00285.2003",
language = "English",
volume = "287",
pages = "L835--L842",
journal = "American Journal of Physiology - Lung Cellular and Molecular Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "4 31-4",

}

Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells. / Kim, Chang Hoon; Kim, Sung Shik; Choi, Jae Young; Shin, Ji Hyun; Kim, Jin Young; Namkung, Wan; Lee, Jeung Gweon; Lee, Min Goo; Yoon, Joo Heon.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 287, No. 4 31-4, 01.10.2004, p. L835-L842.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells

AU - Kim, Chang Hoon

AU - Kim, Sung Shik

AU - Choi, Jae Young

AU - Shin, Ji Hyun

AU - Kim, Jin Young

AU - Namkung, Wan

AU - Lee, Jeung Gweon

AU - Lee, Min Goo

AU - Yoon, Joo Heon

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency, and mucin secretion. Therefore, this study aims to investigate the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells, In RT-PCR, the mRNAs for several P2X (P2X3, P2X 4, P2X7) and P2Y (P2Y1, P2Y2, (P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring intracellular calcium concentration ([Ca 2+]i) increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of αβ-methylene ATP and 2-methylthio-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase is concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies. In addition, the results with 2,3-O-(4-benzoyl)- benzoyl-ATP indicate that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y 2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.

AB - Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency, and mucin secretion. Therefore, this study aims to investigate the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells, In RT-PCR, the mRNAs for several P2X (P2X3, P2X 4, P2X7) and P2Y (P2Y1, P2Y2, (P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring intracellular calcium concentration ([Ca 2+]i) increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of αβ-methylene ATP and 2-methylthio-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase is concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies. In addition, the results with 2,3-O-(4-benzoyl)- benzoyl-ATP indicate that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y 2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.

UR - http://www.scopus.com/inward/record.url?scp=4544377752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4544377752&partnerID=8YFLogxK

U2 - 10.1152/ajplung.00285.2003

DO - 10.1152/ajplung.00285.2003

M3 - Article

C2 - 15208093

AN - SCOPUS:4544377752

VL - 287

SP - L835-L842

JO - American Journal of Physiology - Lung Cellular and Molecular Physiology

JF - American Journal of Physiology - Lung Cellular and Molecular Physiology

SN - 1040-0605

IS - 4 31-4

ER -