Mesenchymal Stem Cells Isolated from Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway

Anwar Hossain, Joy Gumin, Feng Gao, Javier Figueroa, Naoki Shinojima, Tatsuya Takezaki, Waldemar Priebe, Diana Villarreal, Seok-Gu Kang, Celine Joyce, Erik Sulman, Qianghu Wang, Frank C. Marini, Michael Andreeff, Howard Colman, Frederick F. Lang

Research output: Contribution to journalArticle

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Abstract

Although mesenchymal stem cells (MSCs) have been implicated as stromal components of several cancers, their ultimate contribution to tumorigenesis and their potential to drive cancer stem cells, particularly in the unique microenvironment of human brain tumors, remain largely undefined. Consequently, using established criteria, we isolated glioma-associated-human MSCs (GA-hMSCs) from fresh human glioma surgical specimens for the first time. We show that these GA-hMSCs are nontumorigenic stromal cells that are phenotypically similar to prototypical bone marrow-MSCs. Low-passage genomic sequencing analyses comparing GA-hMSCs with matched tumor-initiating glioma stem cells (GSCs) suggest that most GA-hMSCs (60%) are normal cells recruited to the tumor (group 1 GA-hMSCs), although, rarely (10%), GA-hMSCs may differentiate directly from GSCs (group 2 GA-hMSCs) or display genetic patterns intermediate between these groups (group 3 GA-hMSCs). Importantly, GA-hMSCs increase proliferation and self-renewal of GSCs in vitro and enhance GSC tumorigenicity and mesenchymal features in vivo, confirming their functional significance within the GSC niche. These effects are mediated by GA-hMSC-secreted interleukin-6, which activates STAT3 in GSCs. Our results establish GA-hMSCs as a potentially new stromal component of gliomas that drives the aggressiveness of GSCs, and point to GA-hMSCs as a novel therapeutic target within gliomas. Stem Cells 2015;33:2400-2415

Original languageEnglish
Pages (from-to)2400-2415
Number of pages16
JournalStem Cells
Volume33
Issue number8
DOIs
Publication statusPublished - 2015 Aug 1

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Mesenchymal Stromal Cells
Glioma
Interleukin-6
Stem Cells
Stem Cell Niche
Neoplasms
Neoplastic Stem Cells

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Hossain, Anwar ; Gumin, Joy ; Gao, Feng ; Figueroa, Javier ; Shinojima, Naoki ; Takezaki, Tatsuya ; Priebe, Waldemar ; Villarreal, Diana ; Kang, Seok-Gu ; Joyce, Celine ; Sulman, Erik ; Wang, Qianghu ; Marini, Frank C. ; Andreeff, Michael ; Colman, Howard ; Lang, Frederick F. / Mesenchymal Stem Cells Isolated from Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. In: Stem Cells. 2015 ; Vol. 33, No. 8. pp. 2400-2415.
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abstract = "Although mesenchymal stem cells (MSCs) have been implicated as stromal components of several cancers, their ultimate contribution to tumorigenesis and their potential to drive cancer stem cells, particularly in the unique microenvironment of human brain tumors, remain largely undefined. Consequently, using established criteria, we isolated glioma-associated-human MSCs (GA-hMSCs) from fresh human glioma surgical specimens for the first time. We show that these GA-hMSCs are nontumorigenic stromal cells that are phenotypically similar to prototypical bone marrow-MSCs. Low-passage genomic sequencing analyses comparing GA-hMSCs with matched tumor-initiating glioma stem cells (GSCs) suggest that most GA-hMSCs (60{\%}) are normal cells recruited to the tumor (group 1 GA-hMSCs), although, rarely (10{\%}), GA-hMSCs may differentiate directly from GSCs (group 2 GA-hMSCs) or display genetic patterns intermediate between these groups (group 3 GA-hMSCs). Importantly, GA-hMSCs increase proliferation and self-renewal of GSCs in vitro and enhance GSC tumorigenicity and mesenchymal features in vivo, confirming their functional significance within the GSC niche. These effects are mediated by GA-hMSC-secreted interleukin-6, which activates STAT3 in GSCs. Our results establish GA-hMSCs as a potentially new stromal component of gliomas that drives the aggressiveness of GSCs, and point to GA-hMSCs as a novel therapeutic target within gliomas. Stem Cells 2015;33:2400-2415",
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Hossain, A, Gumin, J, Gao, F, Figueroa, J, Shinojima, N, Takezaki, T, Priebe, W, Villarreal, D, Kang, S-G, Joyce, C, Sulman, E, Wang, Q, Marini, FC, Andreeff, M, Colman, H & Lang, FF 2015, 'Mesenchymal Stem Cells Isolated from Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway', Stem Cells, vol. 33, no. 8, pp. 2400-2415. https://doi.org/10.1002/stem.2053

Mesenchymal Stem Cells Isolated from Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. / Hossain, Anwar; Gumin, Joy; Gao, Feng; Figueroa, Javier; Shinojima, Naoki; Takezaki, Tatsuya; Priebe, Waldemar; Villarreal, Diana; Kang, Seok-Gu; Joyce, Celine; Sulman, Erik; Wang, Qianghu; Marini, Frank C.; Andreeff, Michael; Colman, Howard; Lang, Frederick F.

In: Stem Cells, Vol. 33, No. 8, 01.08.2015, p. 2400-2415.

Research output: Contribution to journalArticle

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AU - Hossain, Anwar

AU - Gumin, Joy

AU - Gao, Feng

AU - Figueroa, Javier

AU - Shinojima, Naoki

AU - Takezaki, Tatsuya

AU - Priebe, Waldemar

AU - Villarreal, Diana

AU - Kang, Seok-Gu

AU - Joyce, Celine

AU - Sulman, Erik

AU - Wang, Qianghu

AU - Marini, Frank C.

AU - Andreeff, Michael

AU - Colman, Howard

AU - Lang, Frederick F.

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AB - Although mesenchymal stem cells (MSCs) have been implicated as stromal components of several cancers, their ultimate contribution to tumorigenesis and their potential to drive cancer stem cells, particularly in the unique microenvironment of human brain tumors, remain largely undefined. Consequently, using established criteria, we isolated glioma-associated-human MSCs (GA-hMSCs) from fresh human glioma surgical specimens for the first time. We show that these GA-hMSCs are nontumorigenic stromal cells that are phenotypically similar to prototypical bone marrow-MSCs. Low-passage genomic sequencing analyses comparing GA-hMSCs with matched tumor-initiating glioma stem cells (GSCs) suggest that most GA-hMSCs (60%) are normal cells recruited to the tumor (group 1 GA-hMSCs), although, rarely (10%), GA-hMSCs may differentiate directly from GSCs (group 2 GA-hMSCs) or display genetic patterns intermediate between these groups (group 3 GA-hMSCs). Importantly, GA-hMSCs increase proliferation and self-renewal of GSCs in vitro and enhance GSC tumorigenicity and mesenchymal features in vivo, confirming their functional significance within the GSC niche. These effects are mediated by GA-hMSC-secreted interleukin-6, which activates STAT3 in GSCs. Our results establish GA-hMSCs as a potentially new stromal component of gliomas that drives the aggressiveness of GSCs, and point to GA-hMSCs as a novel therapeutic target within gliomas. Stem Cells 2015;33:2400-2415

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