Mesoporous platinum electrodes for amperometric determination of sugars with anion exchange chromatography

Jee Hoon Han, Han Nim Choi, Sejin Park, Taek Dong Chung, Won Yong Lee

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Abstract

A simple amperometric detection (AD) method based on mesoporous platinum (Pt) electrodes has been developed for the determination of sugars (glucose, fructose, and sucrose) with high-performance anion-exchange chromatography (HPAEC). The amperometric detection is based on the direct oxidation of sugars on mesoporous Pt films formed on a gold electrode. The mesoporous Pt electrode (roughness factor of 243) sensitively responded to glucose, fructose, and sucrose in 80 mM sodium hydroxide solution as an alkaline mobile-phase for HPAEC. Under the optimum conditions, the limits of detection (S/N = 3) in these sugars were 0.24, 0.29, and 1.8 mM, for glucose, fructose, and sucrose, respectively. The reproducibility (relative standard deviation) of the measurements was less than 3.5%. The present method was applied to the determination of sugars in apple juice. The recoveries for all sugars ranged from 97 to 99%.

Original languageEnglish
Pages (from-to)995-1000
Number of pages6
JournalAnalytical Sciences
Volume26
Issue number9
DOIs
Publication statusPublished - 2010

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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