Mesoporous TiO2 as a nanostructured substrate for cell culture and cell patterning

Sangphil Park, Sung Hoon Ahn, Hyun Jong Lee, Ui Seok Chung, Jong Hak Kim, Won Gun Koh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the behaviors of mammalian cells such as adhesion, morphology and proliferation on mesoporous TiO2-coated glass slides using NIH-3T3 fibroblasts as a model cell. A sol-gel process using amphiphilic poly(vinyl chloride) (PVC)-g-poly(oxyethylene methacrylate) (POEM) graft copolymer as a template produced defect or crack-free and homogeneous mesoporous TiO2 films over a large area with high porosity and good connectivity. Cells grown on the mesoporous TiO2 surfaces exhibited less spreading and had more filopodia than cells on the flat glass slides. The nanotopographical cues from the mesoporous TiO2 resulted in the formation of more focal adhesions, promoting cell adhesion and proliferation without decreasing cell viability and functionality. We also demonstrated the capability of controlling spatial placement of cells onto chemically and topologically structured templates by fabricating poly(ethylene glycol) (PEG) hydrogel micropatterns on mesoporous TiO2 films. Because a hydrogel precursor solution could infiltrate and become crosslinked within the multilayered mesoporous TiO2 films, the resultant hydrogel micropatterns were firmly anchored on the substrate without the use of adhesion-promoting monolayers. While nanoscale topographic cues from mesoporous structure contributed to enhancement of cellular behaviors, different chemistry between cell-repelling PEG hydrogel and cell-adhesive mesoporous region facilitated confinement of cells on the micrometer scale. This study suggests that developed mesoporous TiO2 films hold high potential for bioapplications showing high biocompatibility as surface coating materials for implants or as biomimetic platforms for cell patterning.

Original languageEnglish
Pages (from-to)23673-23680
Number of pages8
JournalRSC Advances
Volume3
Issue number45
DOIs
Publication statusPublished - 2013 Dec 7

Fingerprint

Hydrogel
Cell culture
Hydrogels
Polyethylene glycols
Cells
Adhesion
Substrates
Vinyl Chloride
Glass
Methacrylates
Graft copolymers
Cell adhesion
Cell proliferation
Biomimetics
Fibroblasts
Biocompatibility
Polyvinyl Chloride
Sol-gel process
Monolayers
Adhesives

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Park, Sangphil ; Ahn, Sung Hoon ; Lee, Hyun Jong ; Chung, Ui Seok ; Kim, Jong Hak ; Koh, Won Gun. / Mesoporous TiO2 as a nanostructured substrate for cell culture and cell patterning. In: RSC Advances. 2013 ; Vol. 3, No. 45. pp. 23673-23680.
@article{3429677e2cc4428da722d1e9d24a2956,
title = "Mesoporous TiO2 as a nanostructured substrate for cell culture and cell patterning",
abstract = "We investigated the behaviors of mammalian cells such as adhesion, morphology and proliferation on mesoporous TiO2-coated glass slides using NIH-3T3 fibroblasts as a model cell. A sol-gel process using amphiphilic poly(vinyl chloride) (PVC)-g-poly(oxyethylene methacrylate) (POEM) graft copolymer as a template produced defect or crack-free and homogeneous mesoporous TiO2 films over a large area with high porosity and good connectivity. Cells grown on the mesoporous TiO2 surfaces exhibited less spreading and had more filopodia than cells on the flat glass slides. The nanotopographical cues from the mesoporous TiO2 resulted in the formation of more focal adhesions, promoting cell adhesion and proliferation without decreasing cell viability and functionality. We also demonstrated the capability of controlling spatial placement of cells onto chemically and topologically structured templates by fabricating poly(ethylene glycol) (PEG) hydrogel micropatterns on mesoporous TiO2 films. Because a hydrogel precursor solution could infiltrate and become crosslinked within the multilayered mesoporous TiO2 films, the resultant hydrogel micropatterns were firmly anchored on the substrate without the use of adhesion-promoting monolayers. While nanoscale topographic cues from mesoporous structure contributed to enhancement of cellular behaviors, different chemistry between cell-repelling PEG hydrogel and cell-adhesive mesoporous region facilitated confinement of cells on the micrometer scale. This study suggests that developed mesoporous TiO2 films hold high potential for bioapplications showing high biocompatibility as surface coating materials for implants or as biomimetic platforms for cell patterning.",
author = "Sangphil Park and Ahn, {Sung Hoon} and Lee, {Hyun Jong} and Chung, {Ui Seok} and Kim, {Jong Hak} and Koh, {Won Gun}",
year = "2013",
month = "12",
day = "7",
doi = "10.1039/c3ra45136d",
language = "English",
volume = "3",
pages = "23673--23680",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "45",

}

Mesoporous TiO2 as a nanostructured substrate for cell culture and cell patterning. / Park, Sangphil; Ahn, Sung Hoon; Lee, Hyun Jong; Chung, Ui Seok; Kim, Jong Hak; Koh, Won Gun.

In: RSC Advances, Vol. 3, No. 45, 07.12.2013, p. 23673-23680.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mesoporous TiO2 as a nanostructured substrate for cell culture and cell patterning

AU - Park, Sangphil

AU - Ahn, Sung Hoon

AU - Lee, Hyun Jong

AU - Chung, Ui Seok

AU - Kim, Jong Hak

AU - Koh, Won Gun

PY - 2013/12/7

Y1 - 2013/12/7

N2 - We investigated the behaviors of mammalian cells such as adhesion, morphology and proliferation on mesoporous TiO2-coated glass slides using NIH-3T3 fibroblasts as a model cell. A sol-gel process using amphiphilic poly(vinyl chloride) (PVC)-g-poly(oxyethylene methacrylate) (POEM) graft copolymer as a template produced defect or crack-free and homogeneous mesoporous TiO2 films over a large area with high porosity and good connectivity. Cells grown on the mesoporous TiO2 surfaces exhibited less spreading and had more filopodia than cells on the flat glass slides. The nanotopographical cues from the mesoporous TiO2 resulted in the formation of more focal adhesions, promoting cell adhesion and proliferation without decreasing cell viability and functionality. We also demonstrated the capability of controlling spatial placement of cells onto chemically and topologically structured templates by fabricating poly(ethylene glycol) (PEG) hydrogel micropatterns on mesoporous TiO2 films. Because a hydrogel precursor solution could infiltrate and become crosslinked within the multilayered mesoporous TiO2 films, the resultant hydrogel micropatterns were firmly anchored on the substrate without the use of adhesion-promoting monolayers. While nanoscale topographic cues from mesoporous structure contributed to enhancement of cellular behaviors, different chemistry between cell-repelling PEG hydrogel and cell-adhesive mesoporous region facilitated confinement of cells on the micrometer scale. This study suggests that developed mesoporous TiO2 films hold high potential for bioapplications showing high biocompatibility as surface coating materials for implants or as biomimetic platforms for cell patterning.

AB - We investigated the behaviors of mammalian cells such as adhesion, morphology and proliferation on mesoporous TiO2-coated glass slides using NIH-3T3 fibroblasts as a model cell. A sol-gel process using amphiphilic poly(vinyl chloride) (PVC)-g-poly(oxyethylene methacrylate) (POEM) graft copolymer as a template produced defect or crack-free and homogeneous mesoporous TiO2 films over a large area with high porosity and good connectivity. Cells grown on the mesoporous TiO2 surfaces exhibited less spreading and had more filopodia than cells on the flat glass slides. The nanotopographical cues from the mesoporous TiO2 resulted in the formation of more focal adhesions, promoting cell adhesion and proliferation without decreasing cell viability and functionality. We also demonstrated the capability of controlling spatial placement of cells onto chemically and topologically structured templates by fabricating poly(ethylene glycol) (PEG) hydrogel micropatterns on mesoporous TiO2 films. Because a hydrogel precursor solution could infiltrate and become crosslinked within the multilayered mesoporous TiO2 films, the resultant hydrogel micropatterns were firmly anchored on the substrate without the use of adhesion-promoting monolayers. While nanoscale topographic cues from mesoporous structure contributed to enhancement of cellular behaviors, different chemistry between cell-repelling PEG hydrogel and cell-adhesive mesoporous region facilitated confinement of cells on the micrometer scale. This study suggests that developed mesoporous TiO2 films hold high potential for bioapplications showing high biocompatibility as surface coating materials for implants or as biomimetic platforms for cell patterning.

UR - http://www.scopus.com/inward/record.url?scp=84886853049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886853049&partnerID=8YFLogxK

U2 - 10.1039/c3ra45136d

DO - 10.1039/c3ra45136d

M3 - Article

AN - SCOPUS:84886853049

VL - 3

SP - 23673

EP - 23680

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 45

ER -