Metabolic alterations in the bone tissues of aged osteoporotic mice

Miso Nam, Jeong Eun Huh, Min Sun Kim, Do Hyun Ryu, Jihyeong Park, Han Sung Kim, Soo Young Lee, Geum Sook Hwang

Research output: Contribution to journalArticle

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Abstract

Age-related osteoporosis is characterized by reduced bone mineralization and reduced bone strength, which increases the risk of fractures. We examined metabolic changes associated with age-related bone loss by profiling lipids and polar metabolites in tibia and femur bone tissues from young (5 months old) and old (28 months old) male C57BL/6J mice using ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry. Partial least-squares discriminant analysis showed clear differences in metabolite levels in bone tissues of young and old mice. We identified 93 lipid species, including free fatty acids, sphingolipids, phospholipids, and glycerolipids, that were significantly altered in bone tissues of old mice. In addition, the expression of 26 polar metabolites differed significantly in bone tissues of old mice and young mice. Specifically, uremic toxin metabolite levels (p-cresyl sulfate, hippuric acid, and indoxylsulfate) were higher in bone tissues of old mice than in young mice. The increase in p-cresyl sulfate, hippuric acid, and indoxylsulfate levels were determined using targeted analysis of plasma polar extracts to determine whether these metabolites could serve as potential osteoporosis biomarkers. This study demonstrates that LC-MS-based global profiling of lipid and polar metabolites can elucidate metabolic changes that occur during age-related bone loss and identify potential biomarkers of osteoporosis.

Original languageEnglish
Article number8127
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Osteoporosis
Bone and Bones
Lipids
Sulfates
Biomarkers
Physiologic Calcification
Sphingolipids
Discriminant Analysis
Least-Squares Analysis
Tibia
Inbred C57BL Mouse
Nonesterified Fatty Acids
Liquid Chromatography
Femur
Mass Spectrometry
Phospholipids
hippuric acid

All Science Journal Classification (ASJC) codes

  • General

Cite this

Nam, M., Huh, J. E., Kim, M. S., Ryu, D. H., Park, J., Kim, H. S., ... Hwang, G. S. (2018). Metabolic alterations in the bone tissues of aged osteoporotic mice. Scientific reports, 8(1), [8127]. https://doi.org/10.1038/s41598-018-26322-7
Nam, Miso ; Huh, Jeong Eun ; Kim, Min Sun ; Ryu, Do Hyun ; Park, Jihyeong ; Kim, Han Sung ; Lee, Soo Young ; Hwang, Geum Sook. / Metabolic alterations in the bone tissues of aged osteoporotic mice. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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Nam, M, Huh, JE, Kim, MS, Ryu, DH, Park, J, Kim, HS, Lee, SY & Hwang, GS 2018, 'Metabolic alterations in the bone tissues of aged osteoporotic mice', Scientific reports, vol. 8, no. 1, 8127. https://doi.org/10.1038/s41598-018-26322-7

Metabolic alterations in the bone tissues of aged osteoporotic mice. / Nam, Miso; Huh, Jeong Eun; Kim, Min Sun; Ryu, Do Hyun; Park, Jihyeong; Kim, Han Sung; Lee, Soo Young; Hwang, Geum Sook.

In: Scientific reports, Vol. 8, No. 1, 8127, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Kim, Han Sung

AU - Lee, Soo Young

AU - Hwang, Geum Sook

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