Metabolic heterogeneity of activated beige/brite adipocytes in inguinal adipose tissue

Yun Hee Lee, Sang Nam Kim, Hyun Jung Kwon, James G. Granneman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sustained β3 adrenergic receptor (ADRB3) activation simultaneously upregulates fatty acid synthesis and oxidation in mouse brown, beige, and white adipose tissues; however, the cellular basis of this dual regulation is not known. Treatment of mice with the ADRB3 agonist CL316,243 (CL) increased expression of fatty acid synthase (FASN) and medium chain acyl-CoA dehydrogenase (MCAD) protein within the same cells in classic brown and white adipose tissues. Surprisingly, in inguinal adipose tissue, CL-upregulated FASN and MCAD in distinct cell populations: high MCAD expression occurred in multilocular adipocytes that co-expressed UCP1+, whereas high FASN expression occurred in paucilocular adipocytes lacking detectable UCP1. Genetic tracing with UCP1-cre, however, indicated nearly half of adipocytes with a history of UCP1 expression expressed high levels of FASN without current expression of UCP1. Global transcriptomic analysis of FACS-isolated adipocytes confirmed the presence of distinct anabolic and catabolic phenotypes, and identified differential expression of transcriptional pathways known to regulate lipid synthesis and oxidation. Surprisingly, paternally-expressed genes of the non-classical gene imprinted network were strikingly enriched in anabolic phenotypes, suggesting possible involvement in maintaining the balance of metabolic phenotypes. The results indicate that metabolic heterogeneity is a distinct property of activated beige/brite adipocytes that might be under epigenetic control.

Original languageEnglish
Article number39794
JournalScientific reports
Volume7
DOIs
Publication statusPublished - 2017 Jan 3

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Fatty Acid Synthases
Groin
Acyl-CoA Dehydrogenase
Adipocytes
Adipose Tissue
White Adipose Tissue
Brown Adipose Tissue
Phenotype
Gene Regulatory Networks
Epigenomics
Adrenergic Receptors
Up-Regulation
Fatty Acids
Lipids
Beige Adipocytes
Population
Genes
Proteins
disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate

All Science Journal Classification (ASJC) codes

  • General

Cite this

Lee, Yun Hee ; Kim, Sang Nam ; Kwon, Hyun Jung ; Granneman, James G. / Metabolic heterogeneity of activated beige/brite adipocytes in inguinal adipose tissue. In: Scientific reports. 2017 ; Vol. 7.
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Metabolic heterogeneity of activated beige/brite adipocytes in inguinal adipose tissue. / Lee, Yun Hee; Kim, Sang Nam; Kwon, Hyun Jung; Granneman, James G.

In: Scientific reports, Vol. 7, 39794, 03.01.2017.

Research output: Contribution to journalArticle

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