Metabolic pathway signatures associated with urinary metabolite biomarkers differentiate bladder cancer patients from healthy controls

Won Tae Kim, Seok Joong Yun, Chunri Yan, Pildu Jeong, Ye Hwan Kim, Il Seok Lee, Ho Won Kang, Sunghyouk Park, Sung Kwon Moon, Yung Hyun Choi, Young Deuk Choi, Isaac Yi Kim, Jayoung Kim, Wun Jae Kim

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Purpose: Our previous high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry study identified bladder cancer (BCA)-specific urine metabolites, including carnitine, acylcarnitines, and melatonin. The objective of the current study was to determine which metabolic pathways are perturbed in BCA, based on our previously identified urinary metabolome. Materials and Methods: A total of 135 primary BCA samples and 26 control tissue samples from healthy volunteers were analyzed. The association between specific urinary metabolites and their related encoding genes was analyzed. Results: Significant alterations in the carnitine-acylcarnitine and tryptophan metabolic pathways were detected in urine specimens from BCA patients compared to those of healthy controls. The expression of eight genes involved in the carnitine-acylcarnitine metabolic pathway (CPT1A, CPT1B, CPT1C, CPT2, SLC25A20, and CRAT) or tryptophan metabolism (TPH1 and IDO1) was assessed by RT-PCR in our BCA cohort (n=135). CPT1B, CPT1C, SLC25A20, CRAT, TPH1, and IOD1 were significantly downregulated in tumor tissues compared to normal bladder tissues (p<0.05 all) of patients with non-muscle invasive BCA, whereas CPT1B, CPT1C, CRAT, and TPH1 were downregulated in those with muscle invasive BCA (p<0.05), with no changes in IDO1 expression. Conclusion: Alterations in the expression of genes associated with the carnitine-acylcarnitine and tryptophan metabolic pathways, which were the most perturbed pathways in BCA, were determined.

Original languageEnglish
Pages (from-to)865-871
Number of pages7
JournalYonsei medical journal
Issue number4
Publication statusPublished - 2016 Jul

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. NRF- 2014R1A2A1A09006983 and no. 2014R1A2A2A04007036) (to W.K.), and by NIH grants R01DK100974 (to J.K.).

Publisher Copyright:
© Yonsei University College of Medicine 2016.

All Science Journal Classification (ASJC) codes

  • Medicine(all)


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