Metabolic phenotyping of human atherosclerotic plaques: Metabolic alterations and their biological relevance in plaque-containing aorta

Sunhee Jung, Suk Won Song, Sak Lee, Se Hoon Kim, Soo jin Ann, Eun Jeong Cheon, Gijong Yi, Eui Young Choi, Seung Hyun Lee, Hyun Chel Joo, Do Hyun Ryu, Sang Hak Lee, Geum Sook Hwang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and aims Atherosclerosis is a chronic inflammatory disease characterized by thickening of the arterial wall. However, a limited number of studies have been conducted on metabolic profiling of human aortic tissue. Methods We applied liquid chromatography/mass spectrometry to perform global and targeted profiling of plaque-containing aortic tissue. The aorta samples included plaque-containing (n = 18) and control plaque-free (n = 24) aortic tissue from patients undergoing aortic surgery. Results The metabolic patterns of atherosclerotic and control vessels were significantly different. Metabolites in the purine and glutathione pathways showed dysregulation of oxidative stress in plaques, and levels of glucosylceramide, tryptophan, and kynurenine, which are related to inflammation, were also altered. Interestingly, an increased level of quinic acid was observed in plaques (p < 0.000), and we demonstrated an inhibitory effect of quinic acid on inflammatory activation and oxidative stress in macrophages. Conclusions Our study provides insight into the disease mechanism and potential markers of atherosclerosis through comprehensive metabolic profiling of human aortic tissue samples containing plaque.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalAtherosclerosis
Volume269
DOIs
Publication statusPublished - 2018 Feb

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Atherosclerotic Plaques
Aorta
Quinic Acid
Atherosclerosis
Oxidative Stress
Glucosylceramides
Kynurenine
Tryptophan
Liquid Chromatography
Glutathione
Mass Spectrometry
Chronic Disease
Macrophages
Inflammation

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Jung, Sunhee ; Song, Suk Won ; Lee, Sak ; Kim, Se Hoon ; Ann, Soo jin ; Cheon, Eun Jeong ; Yi, Gijong ; Choi, Eui Young ; Lee, Seung Hyun ; Joo, Hyun Chel ; Ryu, Do Hyun ; Lee, Sang Hak ; Hwang, Geum Sook. / Metabolic phenotyping of human atherosclerotic plaques : Metabolic alterations and their biological relevance in plaque-containing aorta. In: Atherosclerosis. 2018 ; Vol. 269. pp. 21-28.
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abstract = "Background and aims Atherosclerosis is a chronic inflammatory disease characterized by thickening of the arterial wall. However, a limited number of studies have been conducted on metabolic profiling of human aortic tissue. Methods We applied liquid chromatography/mass spectrometry to perform global and targeted profiling of plaque-containing aortic tissue. The aorta samples included plaque-containing (n = 18) and control plaque-free (n = 24) aortic tissue from patients undergoing aortic surgery. Results The metabolic patterns of atherosclerotic and control vessels were significantly different. Metabolites in the purine and glutathione pathways showed dysregulation of oxidative stress in plaques, and levels of glucosylceramide, tryptophan, and kynurenine, which are related to inflammation, were also altered. Interestingly, an increased level of quinic acid was observed in plaques (p < 0.000), and we demonstrated an inhibitory effect of quinic acid on inflammatory activation and oxidative stress in macrophages. Conclusions Our study provides insight into the disease mechanism and potential markers of atherosclerosis through comprehensive metabolic profiling of human aortic tissue samples containing plaque.",
author = "Sunhee Jung and Song, {Suk Won} and Sak Lee and Kim, {Se Hoon} and Ann, {Soo jin} and Cheon, {Eun Jeong} and Gijong Yi and Choi, {Eui Young} and Lee, {Seung Hyun} and Joo, {Hyun Chel} and Ryu, {Do Hyun} and Lee, {Sang Hak} and Hwang, {Geum Sook}",
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Jung, S, Song, SW, Lee, S, Kim, SH, Ann, SJ, Cheon, EJ, Yi, G, Choi, EY, Lee, SH, Joo, HC, Ryu, DH, Lee, SH & Hwang, GS 2018, 'Metabolic phenotyping of human atherosclerotic plaques: Metabolic alterations and their biological relevance in plaque-containing aorta', Atherosclerosis, vol. 269, pp. 21-28. https://doi.org/10.1016/j.atherosclerosis.2017.11.034

Metabolic phenotyping of human atherosclerotic plaques : Metabolic alterations and their biological relevance in plaque-containing aorta. / Jung, Sunhee; Song, Suk Won; Lee, Sak; Kim, Se Hoon; Ann, Soo jin; Cheon, Eun Jeong; Yi, Gijong; Choi, Eui Young; Lee, Seung Hyun; Joo, Hyun Chel; Ryu, Do Hyun; Lee, Sang Hak; Hwang, Geum Sook.

In: Atherosclerosis, Vol. 269, 02.2018, p. 21-28.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Metabolic phenotyping of human atherosclerotic plaques

T2 - Metabolic alterations and their biological relevance in plaque-containing aorta

AU - Jung, Sunhee

AU - Song, Suk Won

AU - Lee, Sak

AU - Kim, Se Hoon

AU - Ann, Soo jin

AU - Cheon, Eun Jeong

AU - Yi, Gijong

AU - Choi, Eui Young

AU - Lee, Seung Hyun

AU - Joo, Hyun Chel

AU - Ryu, Do Hyun

AU - Lee, Sang Hak

AU - Hwang, Geum Sook

PY - 2018/2

Y1 - 2018/2

N2 - Background and aims Atherosclerosis is a chronic inflammatory disease characterized by thickening of the arterial wall. However, a limited number of studies have been conducted on metabolic profiling of human aortic tissue. Methods We applied liquid chromatography/mass spectrometry to perform global and targeted profiling of plaque-containing aortic tissue. The aorta samples included plaque-containing (n = 18) and control plaque-free (n = 24) aortic tissue from patients undergoing aortic surgery. Results The metabolic patterns of atherosclerotic and control vessels were significantly different. Metabolites in the purine and glutathione pathways showed dysregulation of oxidative stress in plaques, and levels of glucosylceramide, tryptophan, and kynurenine, which are related to inflammation, were also altered. Interestingly, an increased level of quinic acid was observed in plaques (p < 0.000), and we demonstrated an inhibitory effect of quinic acid on inflammatory activation and oxidative stress in macrophages. Conclusions Our study provides insight into the disease mechanism and potential markers of atherosclerosis through comprehensive metabolic profiling of human aortic tissue samples containing plaque.

AB - Background and aims Atherosclerosis is a chronic inflammatory disease characterized by thickening of the arterial wall. However, a limited number of studies have been conducted on metabolic profiling of human aortic tissue. Methods We applied liquid chromatography/mass spectrometry to perform global and targeted profiling of plaque-containing aortic tissue. The aorta samples included plaque-containing (n = 18) and control plaque-free (n = 24) aortic tissue from patients undergoing aortic surgery. Results The metabolic patterns of atherosclerotic and control vessels were significantly different. Metabolites in the purine and glutathione pathways showed dysregulation of oxidative stress in plaques, and levels of glucosylceramide, tryptophan, and kynurenine, which are related to inflammation, were also altered. Interestingly, an increased level of quinic acid was observed in plaques (p < 0.000), and we demonstrated an inhibitory effect of quinic acid on inflammatory activation and oxidative stress in macrophages. Conclusions Our study provides insight into the disease mechanism and potential markers of atherosclerosis through comprehensive metabolic profiling of human aortic tissue samples containing plaque.

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