Background and aims Atherosclerosis is a chronic inflammatory disease characterized by thickening of the arterial wall. However, a limited number of studies have been conducted on metabolic profiling of human aortic tissue. Methods We applied liquid chromatography/mass spectrometry to perform global and targeted profiling of plaque-containing aortic tissue. The aorta samples included plaque-containing (n = 18) and control plaque-free (n = 24) aortic tissue from patients undergoing aortic surgery. Results The metabolic patterns of atherosclerotic and control vessels were significantly different. Metabolites in the purine and glutathione pathways showed dysregulation of oxidative stress in plaques, and levels of glucosylceramide, tryptophan, and kynurenine, which are related to inflammation, were also altered. Interestingly, an increased level of quinic acid was observed in plaques (p < 0.000), and we demonstrated an inhibitory effect of quinic acid on inflammatory activation and oxidative stress in macrophages. Conclusions Our study provides insight into the disease mechanism and potential markers of atherosclerosis through comprehensive metabolic profiling of human aortic tissue samples containing plaque.
|Number of pages||8|
|Publication status||Published - 2018 Feb|
Bibliographical noteFunding Information:
This study was supported by the National Research Foundation of Korea ( 2013M3A9B6046418 and 2015M3A6A4076702 ), the National Research Council of Science and Technology ( CAP-2-KBSI ) and the Korea Basic Science Institute ( T37415 ). We are grateful to Jiyeong Jeong, RN, for her excellent assistance with clinical data collection and patient care.
© 2017 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine