Metabolic profiling of human gliomas assessed with NMR

Jung Eun Lee, Sin Soo Jeun, Se Hoon Kim, Chang Young Yoo, Hyeon Man Baek, Seung Ho Yang

Research output: Contribution to journalArticle

Abstract

Little is known about the underlying metabolic alterations of gliomas. The objective of this study was to analyze metabolomic profiles of gliomas diagnosed according to revised WHO classification to demonstrate metabolic signatures beyond isocitrate dehydrogenase (IDH) 1/2 mutation. 1H NMR spectroscopy of tumor extracts was performed to analyze brain tumor metabolism. We detected 46 metabolites including 2-hydroxyglutarate from human brain tumors. Metabolic profiles obtained were analyzed using multivariate analysis and MetaboAnalyst 3.0, a pathway analysis tool. We found that lactate, glutamate, alanine, glutamine, 2-hydroxglutarate, serine, O-phosphocholine, glycine, glycerol, myo-inositol, aspartate, leucine, threonine, creatine, and valine had top-ranked VIP scores in metabolic pathway analyses of glioma. Major metabolism pathways perturbed in glioma included alanine/aspartate/glutamate metabolism, glycine/serine/threonine metabolism, pyruvate metabolism, taurine/hypotaurine metabolism, and D-glutamine/D-glutamate metabolism. Altered metabolites were defined between low-grade and high-grade gliomas. We identified metabolomics signatures of gliomas associated with 2-hydroxglutarate and glioma grade. Metabolic approach may lead to metabolomic cluster-precision strategy and development of metabolic anti-glioma therapy in the future.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalJournal of Clinical Neuroscience
Volume68
DOIs
Publication statusPublished - 2019 Oct

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Glioma
Metabolomics
Glutamic Acid
Threonine
Glutamine
Aspartic Acid
Brain Neoplasms
Alanine
Glycine
Serine
Isocitrate Dehydrogenase
Phosphorylcholine
Metabolome
Creatine
Taurine
Valine
Inositol
Metabolic Networks and Pathways
Pyruvic Acid
Leucine

All Science Journal Classification (ASJC) codes

  • Surgery
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

Cite this

Lee, Jung Eun ; Jeun, Sin Soo ; Kim, Se Hoon ; Yoo, Chang Young ; Baek, Hyeon Man ; Yang, Seung Ho. / Metabolic profiling of human gliomas assessed with NMR. In: Journal of Clinical Neuroscience. 2019 ; Vol. 68. pp. 275-280.
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Metabolic profiling of human gliomas assessed with NMR. / Lee, Jung Eun; Jeun, Sin Soo; Kim, Se Hoon; Yoo, Chang Young; Baek, Hyeon Man; Yang, Seung Ho.

In: Journal of Clinical Neuroscience, Vol. 68, 10.2019, p. 275-280.

Research output: Contribution to journalArticle

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