Metabolic reprogramming by the excessive AMPK activation exacerbates antigen-specific memory CD8+ t cell differentiation after acute lymphocytic choriomeningitis virus infection

Jimin Son, Yong Woo Cho, Youn Jung Woo, Young Ae Baek, Eun Jee Kim, Yuri Cho, Joon Ye Kim, Beom Seok Kim, Jason Jungsik Song, Sang Jun Ha

Research output: Contribution to journalArticle

Abstract

During virus infection, T cells must be adapted to activation and lineage differentiation states via metabolic reprogramming. Whereas effector CD8+ T cells preferentially use glycolysis for their rapid proliferation, memory CD8+ T cells utilize oxidative phosphorylation for their homeostatic maintenance. Particularly, enhanced AMP-activated protein kinase (AMPK) activity promotes the memory T cell response through different pathways. However, the level of AMPK activation required for optimal memory T cell differentiation remains unclear. A new metformin derivative, IM156, formerly known as HL156A, has been reported to ameliorate various types of fibrosis and inhibit in vitro and in vivo tumors by inducing AMPK activation more potently than metformin. Here, we evaluated the in vivo effects of IM156 on antigen-specific CD8+ T cells during their effector and memory differentiation after acute lymphocytic choriomeningitis virus infection. Unexpectedly, our results showed that in vivo treatment of IM156 exacerbated the memory differentiation of virus-specific CD8+ T cells, resulting in an increase in short-lived effector cells but decrease in memory precursor effector cells. Thus, IM156 treatment impaired the function of virus-specific memory CD8+ T cells, indicating that excessive AMPK activation weakens memory T cell differentiation, thereby suppressing recall immune responses. This study suggests that metabolic reprogramming of antigen-specific CD8+ T cells by regulating the AMPK pathway should be carefully performed and managed to improve the efficacy of T cell vaccine.

Original languageEnglish
Article numbere11
JournalImmune Network
Volume19
Issue number2
DOIs
Publication statusPublished - 2019 Apr

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Lymphocytic choriomeningitis virus
AMP-Activated Protein Kinases
Virus Diseases
Cell Differentiation
T-Lymphocytes
Antigens
CD8 Antigens
Metformin
Viruses
Oxidative Phosphorylation
Glycolysis
Fibrosis
Vaccines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Son, Jimin ; Cho, Yong Woo ; Woo, Youn Jung ; Baek, Young Ae ; Kim, Eun Jee ; Cho, Yuri ; Kim, Joon Ye ; Kim, Beom Seok ; Song, Jason Jungsik ; Ha, Sang Jun. / Metabolic reprogramming by the excessive AMPK activation exacerbates antigen-specific memory CD8+ t cell differentiation after acute lymphocytic choriomeningitis virus infection. In: Immune Network. 2019 ; Vol. 19, No. 2.
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Metabolic reprogramming by the excessive AMPK activation exacerbates antigen-specific memory CD8+ t cell differentiation after acute lymphocytic choriomeningitis virus infection. / Son, Jimin; Cho, Yong Woo; Woo, Youn Jung; Baek, Young Ae; Kim, Eun Jee; Cho, Yuri; Kim, Joon Ye; Kim, Beom Seok; Song, Jason Jungsik; Ha, Sang Jun.

In: Immune Network, Vol. 19, No. 2, e11, 04.2019.

Research output: Contribution to journalArticle

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AU - Son, Jimin

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AU - Woo, Youn Jung

AU - Baek, Young Ae

AU - Kim, Eun Jee

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AU - Song, Jason Jungsik

AU - Ha, Sang Jun

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