Removal of a side product to overcome unfavorable equilibrium is a prerequisite for the asymmetric amination of ketones using ω-transaminase (ω-TA). Alanine has been preferred as an amino donor because its deamination product (i.e. pyruvate) is easily removable by several enzymatic methods. Here, we demonstrated that the removal of pyruvate by an innate metabolic pathway could afford equilibrium shift of the ω-TA reactions.
Bibliographical noteFunding Information:
This work was supported by the Advanced Biomass R&D Center [grant number ABC-2011-0031358] through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology.
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry