Metabolically driven equilibrium shift of asymmetric amination of ketones by ω-transaminase using alanine as an amino donor

Sang Woo Han; Jong Shik Shin

doi:10.1080/09168451.2014.930328

Metabolically driven equilibrium shift of asymmetric amination of ketones by ω-transaminase using alanine as an amino donor

Sang Woo Han, Jong Shik Shin

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Removal of a side product to overcome unfavorable equilibrium is a prerequisite for the asymmetric amination of ketones using ω-transaminase (ω-TA). Alanine has been preferred as an amino donor because its deamination product (i.e. pyruvate) is easily removable by several enzymatic methods. Here, we demonstrated that the removal of pyruvate by an innate metabolic pathway could afford equilibrium shift of the ω-TA reactions.

Original language	English
Pages (from-to)	1788-1790
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	78
Issue number	10
DOIs	https://doi.org/10.1080/09168451.2014.930328
Publication status	Published - 2014

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center [grant number ABC-2011-0031358] through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology.

Publisher Copyright:
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2014.930328

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AU - Shin, Jong Shik

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AB - Removal of a side product to overcome unfavorable equilibrium is a prerequisite for the asymmetric amination of ketones using ω-transaminase (ω-TA). Alanine has been preferred as an amino donor because its deamination product (i.e. pyruvate) is easily removable by several enzymatic methods. Here, we demonstrated that the removal of pyruvate by an innate metabolic pathway could afford equilibrium shift of the ω-TA reactions.

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Metabolically driven equilibrium shift of asymmetric amination of ketones by ω-transaminase using alanine as an amino donor

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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