Metabolically driven equilibrium shift of asymmetric amination of ketones by ω-transaminase using alanine as an amino donor

Sang Woo Han, Jong Shik Shin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Removal of a side product to overcome unfavorable equilibrium is a prerequisite for the asymmetric amination of ketones using ω-transaminase (ω-TA). Alanine has been preferred as an amino donor because its deamination product (i.e. pyruvate) is easily removable by several enzymatic methods. Here, we demonstrated that the removal of pyruvate by an innate metabolic pathway could afford equilibrium shift of the ω-TA reactions.

Original languageEnglish
Pages (from-to)1788-1790
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number10
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Amination
Ketones
Alanine Transaminase
Pyruvic Acid
Deamination
Metabolic Networks and Pathways
Transaminases
Alanine

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

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