Metabolite profiling of sex developmental steroid conjugates reveals an association between decreased levels of steroid sulfates and adiposity in obese girls

Su Hyeon Lee, Shin Hye Kim, Wonyong Lee, Bong Chul Chung, Mi Jung Park, Man Ho Choi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Free and conjugated steroids coexist in a dynamic equilibrium due to complex biosynthetic and metabolic processes. This may have clinical significance related to various physiological conditions, including sex development involving the reproductive system. Therefore, we performed quantitative profiling of 16 serum steroids conjugated with glucuronic and sulfuric acids using liquid chromatography-mass spectrometry (LC–MS). All steroid conjugates were purified by solid-phase extraction and then separated through a 3-μm particle size C18 column (150 mm × 2.1 mm) at a flow rate of 0.3 mL/min in the negative ionization mode. The LC–MS-based analysis was found to be linear (r2 > 0.99), and all steroid conjugates had a limit-of-quantification (LOQ) of 10 ng/mL, except for cholesterol sulfate and 17β-estradiol-3,17-disulfate (20 ng/mL). The extraction recoveries of all steroid conjugates ranged from 97.9% to 110.7%, while the overall precision (% CV) and accuracy (% bias) ranged from 4.8% to 10.9% and from 94.4% to 112.9% at four different concentrations, respectively. Profiling of steroid conjugates corrected by adiposity revealed decreased levels of steroid sulfates (P < 0.01) in overweight and obese girls compared to normal girls. The suggested technique can be used for evaluating metabolic changes in steroid conjugates and for understanding the pathophysiology and relative contributions of adiposity in childhood obesity.

Original languageEnglish
Pages (from-to)100-109
Number of pages10
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume162
DOIs
Publication statusPublished - 2016 Sep 1

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Adiposity
Metabolites
Sulfates
Steroids
Association reactions
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Sulfuric Acids
Mass Spectrometry
Sexual Development
Pediatric Obesity
Solid Phase Extraction
Particle Size
Ionization
Particle size
Flow rate
Recovery
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Metabolite profiling of sex developmental steroid conjugates reveals an association between decreased levels of steroid sulfates and adiposity in obese girls",
abstract = "Free and conjugated steroids coexist in a dynamic equilibrium due to complex biosynthetic and metabolic processes. This may have clinical significance related to various physiological conditions, including sex development involving the reproductive system. Therefore, we performed quantitative profiling of 16 serum steroids conjugated with glucuronic and sulfuric acids using liquid chromatography-mass spectrometry (LC–MS). All steroid conjugates were purified by solid-phase extraction and then separated through a 3-μm particle size C18 column (150 mm × 2.1 mm) at a flow rate of 0.3 mL/min in the negative ionization mode. The LC–MS-based analysis was found to be linear (r2 > 0.99), and all steroid conjugates had a limit-of-quantification (LOQ) of 10 ng/mL, except for cholesterol sulfate and 17β-estradiol-3,17-disulfate (20 ng/mL). The extraction recoveries of all steroid conjugates ranged from 97.9{\%} to 110.7{\%}, while the overall precision ({\%} CV) and accuracy ({\%} bias) ranged from 4.8{\%} to 10.9{\%} and from 94.4{\%} to 112.9{\%} at four different concentrations, respectively. Profiling of steroid conjugates corrected by adiposity revealed decreased levels of steroid sulfates (P < 0.01) in overweight and obese girls compared to normal girls. The suggested technique can be used for evaluating metabolic changes in steroid conjugates and for understanding the pathophysiology and relative contributions of adiposity in childhood obesity.",
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Metabolite profiling of sex developmental steroid conjugates reveals an association between decreased levels of steroid sulfates and adiposity in obese girls. / Lee, Su Hyeon; Kim, Shin Hye; Lee, Wonyong; Chung, Bong Chul; Park, Mi Jung; Choi, Man Ho.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 162, 01.09.2016, p. 100-109.

Research output: Contribution to journalArticle

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